Construction of artificial metabolic pathway that is comprised of multiple genes

2015 Fiscal Year Final Research Report

• Project Area: Synthetic biology for the comprehension of biomolecular networks
• Project/Area Number: 23119004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Keio University
• Principal Investigator: TSUGE Kenji 慶應義塾大学, 政策・メディア研究科, 特任講師 (70399690)
• Co-Investigator(Renkei-kenkyūsha): ITAYA Mitsuhiro 慶應義塾大学, 政策・メディア研究科, 教授 (60374013)
• Research Collaborator: YOSHIZUMI Takeshi
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: ゲノムデザイン / 遺伝集積 / オペロン / 遺伝子発現制御 / 代謝工学

Outline of Final Research Achievements

In this study, we tried to elucidate how to design artificial polycistronic operon, especially in terms of gene order in the operon. We used carotenoid and anthocyanin biosynthetic pathway which don't exist in Escherichia coli.
As a result, we succeeded in construction of artificial carotenoid operon plasmid that is fully responsible for carotenoid production starting from glucose. We also succeeded in construction of artificial anthocyanin operon that is designed using information of optimal ration of responsible 4 enzymes by in vitro experiment. Through these case studies, we propose possible rule for construction of artificial operon.

Free Research Field

ゲノムデザイン学