Development of microfluidic platforms for functional evaluation of artificial gene networks

2015 Fiscal Year Final Research Report

• Project Area: Synthetic biology for the comprehension of biomolecular networks
• Project/Area Number: 23119006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: The University of Tokyo
• Principal Investigator: Rondelez Yannick 東京大学, 生産技術研究所, 准教授 (10548770)
• Co-Investigator(Renkei-kenkyūsha): FUJII TERUO 東京大学, 生産技術研究所, 教授 (30251474) ; KANEDA SHOHEI 東京大学, 生産技術研究所, 助教 (10542467)
• Project Period (FY): 2011-04-01 – 2016-03-31
• Keywords: マイクロ・ナノデバイス / マイクロ液滴 / マイクロ流体デバイス / 合成生物学 / In vitro反応ネットワーク / DNA toolbox

Outline of Final Research Achievements

To establish fundamental experimental technologies to support development of the research area of synthetic biology, we developed microfluidic platform can be used for evaluation for functions of artificial gene networks and artificial metabolic pathways. In particular, (1) microchamber-based microfluidic platforms, (2) microdroplet-based microfluidic platform and (3) microfluidic platforms for other groups in this research area. In the development of (2) microdroplet-based microfluidic platform, a microfluidic device can search conditions of concentrations of target molecules in reaction networks in a high-throughput manner (screen ten-thousands conditions of concentrations at once) could be successfully realized.

Free Research Field

In vitro反応ネットワーク