2016 Fiscal Year Annual Research Report
Diagnostic analyses of dynamical interaction of plasma and surface in plasma medicine
Project Area | Plasma medical innovation |
Project/Area Number |
24108002
|
Research Institution | Nagoya University |
Principal Investigator |
堀 勝 名古屋大学, 工学研究科, 教授 (80242824)
|
Co-Investigator(Kenkyū-buntansha) |
加藤 昌志 名古屋大学, 医学系研究科, 教授 (10281073)
秋山 真一 名古屋大学, 医学系研究科, 特任講師 (20500010)
平松 美根男 名城大学, 理工学部, 教授 (50199098)
近藤 博基 名古屋大学, 工学研究科, 准教授 (50345930)
|
Project Period (FY) |
2012-06-28 – 2017-03-31
|
Keywords | プラズマ活性培養液 / プラズマ活性点滴 / 抗腫瘍効果 / シュウ酸カルシウム / 創傷治癒 / 人工脂質二重膜 / 原子間力顕微鏡 |
Outline of Annual Research Achievements |
プラズマ活性培養液の研究に加え、新たなプラズマ活性溶液が発明されプラズマ活性溶液の研究において世界をリードする研究実績を得た。名古屋大学医学部産婦人科、名大病院先端医療臨床研究支援センターとの共同研究によりラズマ照射した点滴(乳酸リンゲル液、酢酸リンゲル液など)プラズマ活性点滴を発明し、プラズマ活性点滴によるグリオブラストーマ培養細胞や卵巣癌培養細胞に対する抗腫瘍効果を見出した。産婦人科、消化器外科との共同研究により腹膜播種モデル動物実験におけるプラズマ活性溶液の抗腫瘍効果を確認した。プラズマ照射した培養液に生じる結晶を解析しシュウ酸カルシウムであることを解明した。名古屋大学医学部生体反応病理学との共同研究により中皮腫培養細胞におけるプラズマ照射による細胞毒性をアスコルビン酸が増強したり減衰したりする条件を見出した。生体反応病理学、産婦人科との共同研究により子宮内膜症モデルマウスにおけるプラズマ照射の有用性が示された。産総研などとの共同研究によりプラズマによる止血の新たなメカニズムが提唱された。また杏林大学秋元らとの共同研究によりプラズマによる創傷治癒過程において形成される膜構造にガレクチン分子が局在することが免疫電顕法により明らかとなった。豊橋技術科学大学手老らとの共同研究により人工脂質二重膜におけるプラズマより生成された中性ラジカル照射によるナノポア構造が原子間力顕微鏡により観察された。以上のように領域横断的な数多くの共同研究によりプラズマが細胞や生体に及ぼす影響やそのメカニズムの解明が爆発的に進められた。
|
Research Progress Status |
28年度が最終年度であるため、記入しない。
|
Strategy for Future Research Activity |
28年度が最終年度であるため、記入しない。
|
Remarks |
2016年度受賞一覧 http://horilab.nuee.nagoya-u.ac.jp/index.php?id=687
|
Research Products
(126 results)
-
-
-
-
-
-
-
-
[Journal Article] Cell survival of glioblastoma grown in medium containing hydrogen peroxide and/or nitrite, or in plasma-activated medium2016
Author(s)
Naoyuki Kurake, Hiromasa Tanaka, Kenji Ishikawa, Takashi Kondo, Keigo Takeda, Hiroki Kondo, Makoto Sekine, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Masaru Hori
-
Journal Title
Archives of Biochemistry and Biophysics
Volume: 605
Pages: 102-108
DOI
Peer Reviewed / Acknowledgement Compliant
-
-
-
-
[Journal Article] Red Blood Cell Coagulation Induced by Low-temperature Plasma Treatment2016
Author(s)
Kenji Miyamoto, Sanae Ikehara, Hikaru Takei, Yoshihiro Akimoto, Hajime Sakakita, Kenji Ishikawa, Masashi Ueda, Jun-ichiro Ikeda, Masahiro Yamagishi, Jaeho Kim, Takashi Yamaguchi, Hayao Nakanishi, Tetsuji Shimizu, Nobuyuki Shimizu, Masaru Hori, Yuzuru Ikehara
-
Journal Title
Archives of Biochemistry and Biophysics
Volume: 605
Pages: 95-101
DOI
Peer Reviewed / Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] プラズマ活性培養液の抗腫瘍効果の増強2017
Author(s)
細井 祐吾,古田 凌, 倉家 尚之, 石川 健治, 橋爪 博司, 田中 宏昌, 竹田 圭吾, 近藤 博基, 関根 誠, 堀 勝
Organizer
第64回応用物理春季学術講演会
Place of Presentation
パシフィコ横浜
Year and Date
2017-03-14 – 2017-03-17
-
-
-
[Presentation] プラズマ活性培養液(PAM)処理グリオーマのメタボロミクス解析2017
Author(s)
倉家 尚之, 田中 宏昌, 石川 健治, 橋爪 博司 中村 香江, 梶山 広明, 吉川 史隆, 水野 正明, 竹田 圭吾, 近藤 博基, 関根 誠, 堀 勝
Organizer
第64回応用物理春季学術講演会
Place of Presentation
パシフィコ横浜
Year and Date
2017-03-14 – 2017-03-17
-
-
-
-
-
-
-
-
-
-
[Presentation] State of The Arts in Plasma Medical Science2017
Author(s)
M.Hori, H.Tanaka, K.Ishikawa, K.Takeda, H.Hashizume, H.Kondo,M.Sekine, M.Mizuno, S.Toyokuni, F.Kikkawa
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research / Invited
-
-
[Presentation] Comparison of Inflammation after Hemostasis with Non-Thermal Plasma and thermal Coagulation by Using Molecular Imagining Technique2017
Author(s)
M.Ueda, D.Yamagami, A.Morioka, K.Watanabe, K.Sano, A.Mori, H.Kimura, H.Saji, H.Sakakita, Y.Ikehara, K.Ishikawa, M.Hori, S.Enomoto
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
-
-
-
[Presentation] Elucidation of the Effects of Plasma-Activated Medium on Supported Lipid Bilayers by employing High-Speed Atomic Force Microscopy2017
Author(s)
T.Tonami, N.Kurake, K.Takeda, K.Ishikawa, H.Hashizume, H.Tanaka, K.Takeda, T.Ohta, H.Kondo, M.Ito, M.Sekine, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
[Presentation] Intracellular Changes of Reactive Oxygen and Nitrogen Species in HeLa Cells Induced by Plasma-Activated Medium2017
Author(s)
R.Furuta, K.Ishikawa, H.Hashizume, H.Tanaka, K.Takeda, T.Ohta, H.Kondo, M.Ito, M.Sekine, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
[Presentation] Antitumor Effect of Culture Medium Irradiated with Non-Equilibrium Atmospheric Pressure Plasmas under Purge of Ambient by N2 and O2 Mixtures2017
Author(s)
Y.Hosoi, R.Furuta, N.Kurake, K.Ishikawa, H.Hashizume,H.Tanaka, K.Takeda, H.Kondo, M.Sekine, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
[Presentation] A Mechanism on Egneration of H2O2 and NO2- in Plasma Activated Medium2017
Author(s)
N.Kurake, H.Tanaka, K.Ishikawa, K.Nakamura, H.Kajiyama, F.Kikkawa, T.Kondo, M.Mizuno,K.Takeda, H.Kondo, M.Sekine, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
[Presentation] Enhancement of Cancer Cell Killing Effect of Hydrogen Peroxide by Nitrite Existence in Plasma Activate Medium2017
Author(s)
N.Kurake, H.Tanaka, K.Ishikawa, K.Nakamura, H.Kajiyama, F.Kikkawa, M.Mizuno, K.Takeda, H.Kondo, M.Sekine, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
-
[Presentation] Dynamic Observations on Morphology of HeLa Cell Membranes in Plasma Activated Medium by high-speed Atomic Force Microscopy2017
Author(s)
S.Yamaoka, Y.Tonami, H.Hashizume, H.Kondo, K.Ishikawa, K.Takeda, H.Tanaka, M.Sekine, M.Ito, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
-
-
-
-
[Presentation] Mechanism of Blood Coagulation Induced by Low Temperature Plasma Treatment2017
Author(s)
Y.Ikehara, S.Ikehara, Y.Akimoto, H.Sakakita, K.Ishiakawa, M.Ueda, J.Ikeda, J.Kim, T.Yamaguchi,H.nakanishi, T.Shimizu, N.Shimizu, M.Hori
Organizer
The 1st International Conference on Plasma medical Science Innovation
Place of Presentation
Nagoya University, Noyori Conference Hall
Year and Date
2017-02-27 – 2017-02-28
Int'l Joint Research
-
-
-
-
[Presentation] 高速原子間力顕微鏡を用いたプラズマ活性培養液内の 活性酸素窒素種による支持脂質二重膜変化の解析2017
Author(s)
戸波卓也, 倉家尚之, 橋爪博司, 近藤博基, 石川健治, 竹田圭吾, 田中宏昌, 関根誠, 伊藤昌文, 手老龍吾, 堀 勝
Organizer
第34回 プラズマプロセシング研究会/第29回 プラズマ材料科学シンポジウム
Place of Presentation
北海道大学・学術交流会館
Year and Date
2017-01-16 – 2017-01-18
Int'l Joint Research
-
[Presentation] プラズマ活性培養液処理をしたHeLa細胞の細胞内応答解析2017
Author(s)
古田 凌, 石川 健治, 橋爪 博司, 田中 宏昌, 竹田 圭吾, 太田 貴之, 近藤 博基, 伊藤 昌文, 関根 誠, 堀 勝
Organizer
第34回 プラズマプロセシング研究会/第29回 プラズマ材料科学シンポジウム
Place of Presentation
北海道大学・学術交流会館
Year and Date
2017-01-16 – 2017-01-18
Int'l Joint Research
-
-
[Presentation] Interaction of Plasma with Cells for Agriculture and Biomedical Applications2017
Author(s)
M.Hori, M.Sekine, K.Ishikawa, H.Kondo, K.Takeda, H,Hashizume, H.Tanaka, F.Kikkawa, M.Mizuno, M.Ito
Organizer
AJC-APSE
Place of Presentation
Nong Lam Univsersity
Year and Date
2017-01-11 – 2017-01-14
Int'l Joint Research / Invited
-
-
[Presentation] Plasma-activated Medium (PAM) and Metabolic Analysis of Glioblastoma (U251SP)2016
Author(s)
Kenji Ishikawa, Naoyuki Kurake, Hiroshi Hashizume, Hiromasa Tanaka, Keigo Takeda, Hiroki Kondo, Makoto Sekine, Takashi Kondo, Shoko Ohnuma, Masashi Kato, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Shinya Toyokuni, and Masaru Hori
Organizer
The 26th annual meeting of MRS-J
Place of Presentation
Yokohama, Japan
Year and Date
2016-12-19 – 2016-12-22
Int'l Joint Research / Invited
-
-
-
-
-
-
[Presentation] Generation of Reactive Species In Medium Irradiated laser-Induced-Plasmas2016
Author(s)
Y.Kurokawa, N.Kurake, K.takeda, K.Ishikawa, H.Hashizume, H.tnaka, H.Kondo, M.Sekine, M.Hori
Organizer
AVS 63rd International Symposium & Exhibition
Place of Presentation
Nashville, Tennessee, US
Year and Date
2016-11-06 – 2016-11-11
Int'l Joint Research
-
-
-
[Presentation] Unobtrusively Medical and Health Monitoring of Older Adults in Every-day Life Mobility with a Vehicle2016
Author(s)
K.Ishikawa, S.Onoshima, H.Yukiwa, H.tanaka,H.Hashizume, Y.Baba, M.Hori, T.Hase, M.Kondo, Y.Hasegawa, M.Mizuno, N. Kihara, K.tatsukoshi, H.Odaka
Organizer
The 3rd International Conference on Univseal Village (UV2016)
Place of Presentation
Nagoya, Japan
Year and Date
2016-10-06 – 2016-10-08
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] Metabolic profiles on glioblastoma (U251SP) modified in plasma-activated medium (PAM) cultivation2016
Author(s)
K.Ishikawa, N.Kurake, H.Tanaka, H.Hashizume, K.Takeda, K.Nakamura, H.Kajiyama, H.Kondo, M.Sekine, M.Kato, M.Mizuno, F.Kikkawa, M.Hori
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research
-
[Presentation] Dynamic behavior of HeLa cells in plasma-activated medium2016
Author(s)
R.Furuta, H.hashizume, K.Ishikawa, H.Tanaka, K.Takeda, T. Ohta, H.Kondo, M.Ito, M.Sekine, M.Hori
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research
-
[Presentation] Mechanisum of blood coagulation induced by plasma treatment in addition to the acceleration of the natural blood coagulation process2016
Author(s)
Y.Ikehara, S.Ikehara, Y,.Akimoto, H.sakakita, K.Ishikawa, M.Ueda, J.Ikeda, J.Kim, Y.Yamaguchi, H.Nakanishi, T.Shimizu, N.Shimizu, M. Hori
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research
-
[Presentation] Plasma-activated medium and cancer2016
Author(s)
H.Tanaka, M.Mizuno, K.Ishikawa, K.Takeda, H.Kondo, H.Hashizume, K.Nakamura, H.Kajiyama, H.Kano, Y.Okazaki, S.Toyokuni, S.Maruyama, F.Kikkawa, M.Hori
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research / Invited
-
-
[Presentation] Comparison of Inflammation after hemostasis with non-thermal plasma or thermal coagulation: A histological and nuclear medical evaluation2016
Author(s)
M. Ueda, D.Yamagami, A.Morioka, K.Watanabe, K.Sano, A.Mori, H.Kimra, H.Saji, H.Sakakita, Y.Ikehara, K.Ishikawa, M.Hori, S.Enomoto
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research
-
-
-
-
-
-
-
-
-
[Presentation] Particulates generation in the plasma activated medium (PAM)2016
Author(s)
N.Kurake, H.Tanaka, K.Ishikawa, H.hashizume, K.Nakamura, H.Kajiyama, F.Kikkawa, T.Kondo, M.Mizuno, K.Takeda, H.Kondo, M.Sekine, M.Hori
Organizer
6th International Conference on Plasma Medicine
Place of Presentation
Bratislava, Slovakia
Year and Date
2016-09-04 – 2016-09-09
Int'l Joint Research
-
-
-
[Presentation] Latent Chemistry in Plasma activated medium (PAM)2016
Author(s)
Kenji Ishikawa, Naoyuki Kurake, Hiromasa Tanaka, Hiroko Mizuno, Hiroshi Hashizume, Keigo Takeda, Hiroki Kondo, Makoto Sekine, Takashi Kondo, Shoko Ohnuma, Masashi Kato, Kae Nakamura, Hiroaki Kajiyama, Fumitaka Kikkawa, Masaaki Mizuno, Masaru Hori
Organizer
20th International Vacuum congress IVC-20
Place of Presentation
Busan, Korea
Year and Date
2016-08-21 – 2016-08-26
Int'l Joint Research
-
-
-
-
-
-
-
-
[Presentation] Impact of Synergism of nitrate and hydrogen Preroxide on cell survivals in plasma-activated-medium(PAM)2016
Author(s)
N. Kurake, H. Tanaka, K. Ishikawa, K. Nakamura, H. Kajiyama, F. Kikkawa, T. Kondo, M. Mizuno, H. Kondo, M. Sekine, M. Hori
Organizer
43rd IEEE International Conference on Plasma Science
Place of Presentation
Banff, Alberta, Canada
Year and Date
2016-06-19 – 2016-06-23
Int'l Joint Research
-
-
-
[Presentation] PLASMATREATMENT INDUCES BLOOD CLOT FORMATION2016
Author(s)
Y. Ikehara, S. Ikehara, T. Yamaguchi, Y. Akimoto, H. Sakakita, J. Kim, K. Ishikawa M. Hori H. Nakanishi N. Shimizu
Organizer
43rd IEEE International Conference on Plasma Science
Place of Presentation
Banff, Alberta, Canada
Year and Date
2016-06-19 – 2016-06-23
Int'l Joint Research / Invited
-
[Presentation] Increase in Galectin expression In Healing Wounded Skin Treated with Low-Temperature Plasma: Comparison with Treatment by Electronical Coagulation2016
Author(s)
Y.Akimoto, S.Ikehara, Y.Yamaguchi, J.Kim, H.Kawakami, N.Shimizu, M.Hori, H.Sakakita, Y.Ikehara
Organizer
43rd IEEE International Conference on Plasma Science
Place of Presentation
Banff, Alberta, Canada
Year and Date
2016-06-19 – 2016-06-23
Int'l Joint Research
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-