2017 Fiscal Year Final Research Report
The role of apical cytoskeletons in transition between centrosomes and ciliary basal bodies.
| Project Area | Cilium-centrosome system regulating biosignal flows |
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| Project/Area Number |
24113002
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Osaka University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
YAMAZAKI Yuji 大阪大学, 大学院生命機能研究科/医学系研究科, 准教授 (80527664)
TAMURA Atsushi 大阪大学, 医学系研究科, 助教 (00362525)
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| Project Period (FY) |
2012-06-28 – 2017-03-31
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| Keywords | シリア / 中心体 / 基底小体 / Odf2変異マウス / subdistal appendages / distal appendages / アピカル３層構造 |
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| Outline of Final Research Achievements |
Ciliogenesis is regulated by context-dependent cellular cues, including some transduced through appendage-like structures on ciliary basal bodies called transition fibers and basal feet. We examined the effects of Odf2 deletion constructs on ciliogenesis in Odf2-knockout F9 cells due to electron microscopy and revealed that during ciliogenesis, the different ODF2 fragments were required to form the distal and subdistal appendages (Da and Sa) in centrosomes which are related to transition fibers and basal feet (Tf and Bf), respectively. The Da and Tf were required for ciliogenesis. The Sa and Bf stabilized the microtubules associated. In the epithelial cell sheets, the apical cytoskeleton were formed differentially from centrosomal microtubules possibly with some relations unknown which stablize ciliogenesis and consisted of three layered structures of actin filaments, intermediate filaments, and microtubules, as revealed by the high voltage electron microscopic tomography.
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| Free Research Field |
細胞生物学
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