2017 Fiscal Year Final Research Report
Toward elucidating mechanisms of centriole formation
Project Area | Cilium-centrosome system regulating biosignal flows |
Project/Area Number |
24113003
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Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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Allocation Type | Single-year Grants |
Review Section |
Biological Sciences
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Research Institution | National Institute of Genetics |
Principal Investigator |
KITAGAWA DAIJU 国立遺伝学研究所, 分子遺伝研究系, 教授 (80605725)
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Co-Investigator(Renkei-kenkyūsha) |
Shiratsuchi Gen 国立遺伝学研究所, 分子遺伝研究系, 博士研究員 (80625533)
Yoshiba Satoko 国立遺伝学研究所, 分子遺伝研究系, 助教 (70642213)
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Project Period (FY) |
2012-06-28 – 2017-03-31
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Keywords | 細胞分裂 / 中心体 / 中心小体複製 / 細胞生物学 |
Outline of Final Research Achievements |
The mechanisms of centrosome duplication have been a long-standing mystery in biology and represent an important open question in biology. Like the genetic material, centrosome duplication occurs once per cell cycle, such that the two resulting centrosomes assemble a bipolar spindle during mitosis, ensuring proper chromosome segregation. We established a molecular basis for the onset of centriole formation by demonstrating that direct association of STIL with Plk4 and subsequent STIL phosphorylation by Plk4 lead to centriolar loading of HsSAS-6 for centriolar cartwheel assembly in human cells. Furthermore, we demonstrated a “swift negative feedback” that the coordinated action of the three key factors can trigger the onset of procentriole formation and, concurrently, ensure formation of a single procentriole per parental centriole. We also demonstrated a mechanism regulating the conversion from premature daughter centrioles to functional mother centrioles in human cells.
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Free Research Field |
細胞生物学
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