Comprehensive omics analysis of plant cell wall formation

2016 Fiscal Year Final Research Report

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 24114002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Nara Institute of Science and Technology
• Principal Investigator: DEMURA Taku 奈良先端科学技術大学院大学, バイオサイエンス研究科, 教授 (40272009)
• Co-Investigator(Kenkyū-buntansha): 倉田 哲也 奈良先端科学技術大学院大学, バイオサイエンス研究科, 准教授 (50360540)
• Co-Investigator(Renkei-kenkyūsha): YONEDA Arata 奈良先端科学技術大学院大学, バイオサイエンス研究科, 助教 (50553715) ; YAMAGUCHI Masatoshi 埼玉大学, 大学院・理工学研究科, 准教授 (20373376) ; NARAMOTO Satoshi 東北大学, 大学院・生命科学研究科, 助教 (30612022)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Keywords: 細胞壁形成 / 道管 / 転写ネットワーク / シロイヌナズナ / ヒメツリガネゴケ / オミクス解析 / ハイドロイド / ステライド

Outline of Final Research Achievements

We performed a series of omics analyses on plant cell wall formation. Transcriptome/metabolome analysis revealed that the primary metabolism is strongly activated during secondary cell wall formation. Also, proteome analysis showed that the amount of several enzymes related to lignin biosynthesis and pectin degradation is drastically changed during secondary cell wall formation. On the analysis of primary cell wall formation, we established a system in which primary cell wall regeneration is induced from protoplasts of Arabidopsis cultured cells and mesophyll cells, using which we showed that KORRIGAN2 protein is closely associated with primary cell wall regeneration through transcriptome analysis and mutant analysis. Furthermore, we succeeded in indicating that in a moss plant, Physcomitrella patens, differentiation of water conducting cells (hydrioids) and supporting cells (stereids) is positively controlled by VNS genes, P. patens homologues of VND7.

Free Research Field

植物生理学、植物分子生物学