Functions and molecular mechanisms of membrane trafficking in biogenesis and functions of cell wall

2016 Fiscal Year Final Research Report

• Project Area: Plant cell wall as information-processing system
• Project/Area Number: 24114003
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: National Institute for Basic Biology (2016); The University of Tokyo (2012-2015)
• Principal Investigator: UEDA Takashi 基礎生物学研究所, 細胞動態研究部門, 教授 (10311333)
• Co-Investigator(Renkei-kenkyūsha): UEMURA Tomohiro 東京大学, 大学院理学系研究科, 助教 (90415092) ; FUJIMOTO Masaru 東京大学, 大学院農学生命研究科, 准教授 (60554475) ; EBINE Kazuo 基礎生物学研究所, 細胞動態研究部門, 助教 (90590399)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Keywords: 膜交通 / 細胞壁 / セルロース合成酵素複合体 / イノシトールリン脂質 / エンドサイトーシス / 液胞 / Rab GTPase

Outline of Final Research Achievements

We analyzed mechanisms of cell wall construction and cell wall functions with various techniques, including super-resolution microscopy, biochemistry, and genetics. We succeeded in fine-resolution imaging of transport of the cellulose synthase complex, which is distinctly dependent on PI3K and PI4K. We also analyzed RABA/RAB11 functions in endocytosis of FLS2 to demonstrate functional diversification of RABA/RAB11 members. Furthermore, we demonstrated that the plant vacuolar transport pathway is distinctly regulated from yeasts and animals, which plays a pivotal role in cell wall integrity. Evolutionary cell biological analyses of the membrane trafficking system using the liverwort, Marchantia polymorpha, is also currently underway.

Free Research Field

植物分子細胞生物学