Mechanism of Mycoplasma gliding

2017 Fiscal Year Final Research Report

• Project Area: Harmonized supramolecular machinery for motility and its diversity
• Project/Area Number: 24117002
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Osaka City University
• Principal Investigator: MIYATA Makoto 大阪市立大学, 大学院理学研究科, 教授 (50209912)
• Co-Investigator(Kenkyū-buntansha): 西坂 崇之 学習院大学, 理学部, 教授 (40359112)
• Co-Investigator(Renkei-kenkyūsha): KODERA Noriyuki 金沢大学, バイオAFM先端研究センター, 准教授 (30584635)
• Project Period (FY): 2012-06-28 – 2017-03-31
• Keywords: 細胞運動 / 力学測定 / 電子顕微鏡 / スピロプラズマ / モリクテス綱 / 病原細菌 / ATP合成酵素 / 遺伝子操作

Outline of Final Research Achievements

Mycoplasma mobile, a pathogenic bacterium of freshwater fish, forms a gliding machinery at a pole and performs 'gliding motility by a unique mechanism. In this study, we clarified the structure of gliding machinery, component proteins, mechanical characters, binding with sialic acid oligosaccharides, coupling with ATP hydrolysis etc, based on the working model of the motility mechanism proposed from previous experimental results, and provided concrete discussion. In addition, we clarified the motility machinery and component proteins, for Mycoplasma pneumoniae and Spiroplasma eriocheiris belonging to the class Mollicutes like M. mobile, and discussed the motility mechanisms.

Free Research Field

細菌学