2016 Fiscal Year Final Research Report
Analysis of enhanceosome that regulates plant growth
| Project Area | Integral understanding of the mechanism of transcription cycle through quantitative, high-resolution approaches |
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| Project/Area Number |
24118004
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hiroshima University |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
KUSABA MAKOTO 広島大学, 大学院理学研究科, 教授 (20370653)
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| Project Period (FY) |
2012-06-28 – 2017-03-31
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| Keywords | 植物 / 発現制御 / 信号伝達 |
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| Outline of Final Research Achievements |
DELLA proteins are major plant growth repressors. The mechanism of Gibberellin (GA)-dependent transcription has been explained by DELLA-mediated titration of transcriptional activators and their release through the degradation of DELLAs in response to GA. However, the effect of GA on genome-wide expression is predominantly repression, suggesting the existence of unknown mechanisms of GA function. In this study, we identified a DELLA binding transcription factor, GAF1. Furthermore, we found that GAF1 interacts with the corepressor TPR and that DELLAs and TPR act as coactivators and a corepressor of GAF1, respectively. GA converts the GAF1 complex from transcriptional activator to repressor via the degradation of DELLAs. These results indicate that DELLAs turn on or off two sets of GA-regulated genes via dual functions, namely titration and coactivation, providing a mechanism for the integrative regulation of plant growth and GA homeostasis.
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| Free Research Field |
植物生理・分子
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