Development of chemically expanded biomolecular systems with dynamic ordering function

2017 Fiscal Year Final Research Report

• Project Area: Dynamical ordering of biomolecular systems for creation of integrated functions
• Project/Area Number: 25102006
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Science and Engineering
• Research Institution: Japan Advanced Institute of Science and Technology
• Principal Investigator: Hohsaka Takahiro 北陸先端科学技術大学院大学, 先端科学技術研究科, 教授 (30263619)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Keywords: タンパク質 / 非天然アミノ酸 / 光架橋 / 抗体 / 蛍光

Outline of Final Research Achievements

In this study, several attempts were made to detect and control the dynamic ordering of proteins by site-specific introduction of nonnatural amino acids and nonnatural molecules. Throughout this study, unique methods were developed for expression of fluorescent antibodies that can detect antigen-binding as fluorescence change, in vivo expression of proteins containing nonnatural amino acids, molecular evolution of non-natural aminoacyl-tRNA synthetase within liposomes, specific chemical modification of nonnatural amino acid-containing proteins, introduction of nonnatural molecules into proteins, and inter- and intramolecular photocrosslinking of protein-protein interaction and protein conformational change. These achievements based on the incorporation of nonnatural molecules allowed detection and control of the dynamic ordering of proteins in vitro and in vivo.

Free Research Field

生体関連化学