2017 Fiscal Year Final Research Report
Epigenome dynamics and regulation in preimplantation embryos
| Project Area | Analyses and regulation of germline epigenome |
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| Project/Area Number |
25112006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Nagahama Institute of Bio-Science and Technology |
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Principal Investigator |
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| Project Period (FY) |
2013-06-28 – 2018-03-31
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| Keywords | 着床前胚 / 全能性 / Klf17 / Btg4 / Trim61 / Pramef12 |
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| Outline of Final Research Achievements |
In this study, we examined the function of totipotent-cell specific genes in vivo. We found that Klf17 and Btg4 are maternal effect genes required for preimplantation development. We also revealed that Klf17 and Btg4 are involved in zygotic gene activation and maternal RNA degradation, respectively. We also found that Trim61 possess E3 ubiquitin ligase activity, and is maternal-zygotic effect gene required for preimplantation development. In additoin, we revealed that Pramef12 plays important role in differentiation of male primordial germ cells, and is required for spermatogenesis. Furthermore, we defined culture condition for ESCs that leads to increases the population of totipotent cells.
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| Free Research Field |
着床前胚
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