2017 Fiscal Year Final Research Report
Reconstitution in culture of germ cell differentiation in mice
| Project Area | Mechanisms regulating gamete formation in animals |
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| Project/Area Number |
25114006
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Project Period (FY) |
2013-06-28 – 2018-03-31
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| Keywords | 多能性幹細胞 / 卵母細胞 |
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| Outline of Final Research Achievements |
The female germline undergoes a unique sequence of differentiation processes that endows totipotency to the egg. The reconstitution of these events in vitro using pluripotent stem cells is a key achievement in reproductive biology and regenerative medicine. Here, this study achieved successful reconstitution in vitro of the entire process of oogenesis from pluripotent stem cells. Fully potent mature oocytes were generated in culture from embryonic stem cells as well as induced pluripotent stem cells derived from both embryonic fibroblasts and adult tail tip fibroblasts. Moreover, pluripotent stem cell lines were re-derived from the in vitro-generated eggs, thereby reconstituting a female germline cycle in a dish. This culture system will provide a unique platform for elucidating the molecular mechanisms underlying totipotency and clues to the production of oocytes of other mammalian species in culture.
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| Free Research Field |
生殖生物学
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