A multidimensional analysis of chromatin dynamics in living cells by quantitative measurements and reconstruction

2017 Fiscal Year Final Research Report

• Project Area: Dynamic chromatin structure and function
• Project/Area Number: 25116005
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Biological Sciences
• Research Institution: Tokyo Institute of Technology (2014-2017); Osaka University (2013)
• Principal Investigator: Kimura Hiroshi 東京工業大学, 科学技術創成研究院, 教授 (30241392)
• Co-Investigator(Kenkyū-buntansha): 山縣 一夫 近畿大学, 生物理工学部, 准教授 (10361312)
• Project Period (FY): 2013-06-28 – 2018-03-31
• Keywords: クロマチン / ヒストン修飾 / 遺伝子発現制御 / 転写 / エピジェネティクス / 細胞核

Outline of Final Research Achievements

Chromatin dynamics plays an important role in cell regulation through the control of gene expression and genome integrity. This study aimed to reveal the significance of chromatin dynamics by quantitative measurements and reconstruction in living cells. We have developed techniques to monitor histone modification and DNA methylation in living cells, and revealed the function of histone acetylation in gene activation. This study greatly contributed to understand the basic mechanism of gene regulation in living cells.

Free Research Field

細胞生物学・エピジェネティクス