2018 Fiscal Year Final Research Report
Visualization and significance of various types of regulated cell death in vivo
| Project Area | Homeostatic Regulation by Various Types of Cell Death |
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| Project/Area Number |
26110005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University (2017-2018)
The University of Tokyo (2014-2016) |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
荒川 聡子 東京医科歯科大学, 難治疾患研究所, 講師 (90415159)
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| Project Period (FY) |
2014-07-10 – 2019-03-31
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| Keywords | 細胞死 / 発生生物学 |
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| Outline of Final Research Achievements |
To elucidate dynamics and significance of various types of regulated cell death in vivo, we developed novel fluorescent probes that allow us to detect pyroptosis and necroptosis in a real-time manner (Cell Rep 2014, Nature Commun 2018). To address the roles and regulation of cell death during mouse development, we analyzed mice deficient for genes regulating apoptosis and revealed involvement of apoptotic machinery in cellular movement during and after neural tube closure, during which a lot of cell death occur with dramatic rewiring of energy metabolic pathway (Dev Cell 2015, Development 2017, Genes Cells 2018, BMC Dev Biol 2018). In addition, we also found that alternative autophagic cell death compensates for apoptosis-deficiency during development (Cell Death Differ 2017).
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| Free Research Field |
発生生物学
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| Academic Significance and Societal Importance of the Research Achievements |
わたしたちの体の中では日々細胞死が生じている。この細胞死を周辺細胞の挙動とともにリアルタイムに可視化・検出することができれば、細胞が死ぬ際の死細胞のふるまいおよび周辺組織への影響を検討することが可能となり、それにより細胞死の生体内での役割についての理解を深めることができる。本研究では、生体内で生じる様々な細胞死のうち、これまでに構築していたアポトーシスに加え、パイロトーシス、ネクロプトーシスといった細胞死のリアルタイム検出系の構築に成功した。さらにそれらを用いて、哺乳類胎児の神経管形成時の細胞死の役割を明らかにした。これらの成果は、炎症や発ガン、胎児奇形の発生等に様々な洞察を与えるものである。
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