2018 Fiscal Year Final Research Report
Functional analyses of physiological function of ncRNA according to the neo-taxonomy
| Project Area | Neo-taxonomy of noncoding RNAs |
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| Project/Area Number |
26113005
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| Research Category |
Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
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| Allocation Type | Single-year Grants |
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| Review Section |
Biological Sciences
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| Research Institution | Hokkaido University (2016-2018)
Institute of Physical and Chemical Research (2014-2015) |
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Principal Investigator |
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| Project Period (FY) |
2014-07-10 – 2019-03-31
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| Keywords | lncRNA / 長鎖ノンコーディングRNA / Neat1 / パラスペックル / RNAseq |
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| Outline of Final Research Achievements |
A number of noncoding RNAs that do not encode proteins are transcribed from the genome. The ncRNAs can be divided into two large groups: "small RNA", which are involved in RNA silencing, and "Long noncoding RNA (lncRNA)", which are more than 200 bases long. It is not clear how many of the lncRNAs actually have physiological functions. We have developed a method to identify novel functional lncRNA candidate genes using the property that known functional lncRNAs are highly efficiently cross-linked to proteins by mild UV irradiation. We also established a technique to analyze the fine structure formed by lncRNA using a super-resolution microscope.
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| Free Research Field |
分子生物学
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| Academic Significance and Societal Importance of the Research Achievements |
lncRNAのうち機能解析が行われたものは全体の1%にも満たず、転写のノイズに過ぎないと考えられているものも多い。また、転写されたRNAではなくその領域をRNAポリメラーゼが通過することに生理的意義があるlncRNAも報告されており、実際にRNA分子が生理機能を持つlncRNAを効率よく同定する方法が求められていた。我々が開発した手法は極めて簡便に機能性lncRNAの候補を同定することが可能であり、今後の効率的な個別の遺伝子の機能解析への道筋をつけることができた。また、lncRNAが形成するサブミクロンオーダーの構造体の内部構造の解析が可能となり、構造に基づいた分子作動機構予測が可能となった。
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