Development of analyzing methods for protein-protein interactions and protein dynamics by means of ODMR and in-cell NMR

2018 Fiscal Year Final Research Report

• Project Area: Novel measurement techniques for visualizing 'live' protein molecules at work
• Project/Area Number: 26119004
• Research Category: Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)
• Allocation Type: Single-year Grants
• Review Section: Complex systems
• Research Institution: Kyoto University
• Principal Investigator: Shirakawa Masahiro 京都大学, 工学研究科, 教授 (00202119)
• Co-Investigator(Renkei-kenkyūsha): TOCHIO Hidehito 京都大学, 理学研究科, 教授 (70336593)
• Project Period (FY): 2014-07-10 – 2019-03-31
• Keywords: ODMR / in-cell NMR / 細胞内蛋白質 / 細胞小器官 / Rheo-NMR

Outline of Final Research Achievements

Methods to create NV centers which are characterized by a crystal lattice vacancy and it adjustments are created. In addition, method targeting the NVC containing ND to biomolecules and technique to measure ODMR of the biomolecule-attached ND have been developed. For protein assemblies (ex. cytoskeltons), by labeling subunit of the assemblies with ND particles, it was possible to characterize the assembly states and mechanism.
We also generated a method to measure NMR spectroscopy of proteins under the presence of rheologic forces --- rheology NMR. The new method is convenient, and applicable to modern NMR machine.

Free Research Field

生物物理学　構造生物化学　分子生物学

Academic Significance and Societal Importance of the Research Achievements

生細胞・生体内での生体高分子の立体構造、集合状態、ダイナミクス等を直接定量的に計測する方法を得つつある。これにより、将来的には、生物の生きた状態を生体分子がいかに成立させているかを、一分子レベルで、またはアンサンブルレベルで可視化・計測することが可能になる。よって、生きた状態を成り立たせる分子論的な必要条件の端緒が見つかると期待される。