1989 Fiscal Year Final Research Report Summary
The Role of Nucleotide Exchange in Regulation of Smooth Muscle Actin Polymerization.
| Project/Area Number |
01044011
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | Joint Research |
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| Research Institution | Obihiro University of Agriculture and Veterinary Medicine. |
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Principal Investigator |
KUWAYAMA Hideto Obihiro University of Agriculture and Veterinary Medicine. Associate Professor., 畜産学部, 助教授 (40125399)
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| Co-Investigator(Kenkyū-buntansha) |
MIKI Masao The University of Sydney. Department of Anatomy. Research Fellow., 解剖学部門, リサーチアソシエイト
DOS REMEDIOS C.G. The University of Sydney. Department of Anatomy. Associate Professor., 解剖学部門, 助教授
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| Project Period (FY) |
1989
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| Keywords | actin / ATP analogue / photoaffinity |
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| Research Abstract |
2-N_3ADP, a photoaffinity ATP analogue, was incorporated into the actin and the influence of the incorporation on the actin function was studied. The replacement of ADP with 2-N_3ADP in F-actin both before and after photo-cross-linking decreased appreciably the actin-activated S1-ATPase activity. Photocrosslinked 2-N_3ADP-F-actin could be depolymerized by dialysis against a solution containing 0.1 mM CaCl_2, 0.1 mM ATP and 1 mM Tris-HCl (pH 8.0). However, once it depolymerized, it lost very quickly the ability to polymerize even in the presence of a sufficient amount of ATP and Ca^<2+>.
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