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1991 Fiscal Year Final Research Report Summary

Molecular Biological Studies on Egg Cytoplasmic Factors That Are Responsible for Regulation of Gene Expression

Research Project

Project/Area Number 01044077
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKyoto University

Principal Investigator

SATOH Noriyuki  Kyoto University, Faculty of Science ; Associate Prof., 理学部, 助教授 (30025481)

Co-Investigator(Kenkyū-buntansha) FEFFERY Will  カリフォルニア大学, デイビス校・ボデガ海岸研究所(米国), 教授
JEFFERY Wiliam R.  Bodega marine Laboratory, University of California U. S. A. ; Professor
Project Period (FY) 1989 – 1991
Keywordscellular Differentiation / Muscle cells / specific genes / Regulation of expression / Egg cytoplasmic factors / Ascidian embryos
Research Abstract

Presumptive muscle cells of early ascidian embryos have an extreme potential of autonomous differentiation. This potential is attributed to prelocalized egg cytoplasmic factors or determinants. The aim of our project is to understand the molecular nature of factors.
The first approach to the problem includes an isolation and characterization of muscle-specific genes and analysis of regulatory factors for the gene expression. We have isolated cDNA clones for myosin-heavy chain and muscle-type actins. Expression of the muscle-specific genes begins around the time of gastrulation, and is restricted to presumptive muscle cells. It has been shown that the ascidian embryo synthesizes several different actin mRNAs. These mRNAs differ from one another in the nucleotide sequences of their 3'non-coding regions, although the sequences of the coding region are almost identical. One of the clones, HrcMA4 contains an open reading frame of 1137 bp and a 100 bp non-coding region followed by a poly (A) tail. Using a specific probe consisting mainly of 3'non-coding region, we have isolated several genomic DNA fragments containing HrMA4 gene. Characterization of the gene shows that HrMA4 is interrupted by two introns ; the position of them resembles to that of vertebrate skeletal muscle actin genes. In addition, it becomes clear that HrMA4 and several other muscle-actin genes form a cluster.
The second approach is to characterize macromolecules of the myoplasm, which is thought to contain the cytoplasmic factors. We have made several monoclonal antibodies which specifically recognize the myoplasmic components. One of the antibodies blocks muscle development when it is injected into fertilized eggs. Characterization of the antigenic polypeptide has revealed that the protein is related to cytoskeletal proteins.

  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] Marikawa,Y.: "Gray and red fragments of the egg of the ascidian Ciona savignyi:preferential development of muscle cells from gray fragments" Develop.Growth Differ.33. 307-316 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusakabe,T.: "Temporal and spatial expression of a muscleactin gene during embryogenesis of the ascidian Halocynthia roretzi" Develop.Growth Differ.33. 227-234 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makabe,K.W.: "Specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryo" Roux's Arch.Dev.Biol.199. 307-313 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh,N.: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" Int.Rev.Cytol.122. 221-258 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makabe,K.W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells." Zool.Sci.9. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kusakabe,T.: "Organization and structure of muscle-type actin genes in the ascidian Halocynthia roretzi"

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh,N.: "Frontiers in Muscle Research" Excerpta Medica, 450 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 佐藤 矩行: "発生・分化の遺伝子的背景" 東京大学出版会, 288 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Satoh, Noriyuki: "Cellular and molecular mechanisms of muscle cell differentiation in ascidian embryos" Intern. Review Cytology. 122. 221-258 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makabe, Kazuhiro W.: "specific expression of myosin heavy chain gene in muscle lineage cells of the ascidian embryos" Roux's Arch. Develop. Biol.199. 307-313 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kusakabe, Takehiro: "Temporal and spatial expression of a muscle actin gene during embryogenesis of the ascidian Halocynthia roretzi" Develop. Growth Differ.33. 227-234 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Marikawa, Yusuke: "Gray and red fragments of the egg of the ascidian Ciona savignyi : Preferential development of muscle cells from gray fragments" Develop. Growth Differ.33. 307-316 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makabe, Kazuhiro W.: "Failure of muscle myosin heavy-chain gene expression in quarter ascidian embryos developed from the secondary muscle lineage cells." Zool. Sci.9. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, Noriyuki: Excerpta Medica. "frontiers in Muscle Research", 169-178 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Satoh, Noriyuki: Tokyo University Press. "Genetic Background of Development and Differentiation", 89-103 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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