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1989 Fiscal Year Final Research Report Summary

Mutagenesis Studies on Ionic Channels

Research Project

Project/Area Number 01044079
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionJoint Research
Research InstitutionKyoto University

Principal Investigator

NUMA Shosaku  Kyoto University Faculty of Medicine, 医学部, 教授 (50025516)

Co-Investigator(Kenkyū-buntansha) KONNO Takashi  Max-Planck-Institut fur medizinische Forschung Abteilung Zellphysiologie, プランク医学研究所・細胞生理学部門, 研究員
SAKMANN Bert  Max-Planck-Institut fur medizinische Forschung Abteilung Zellphysiologie, プランク医学研究所・細胞生理学部門, 部長
FUKUDA Kazuhiko  Kyoto University Faculty of Medicine, 医学部, 助手 (90199224)
TANABE Tsutomu  Kyoto University Faculty of Medicine, 医学部, 助手 (70183069)
IMOTO Keiji  Kyoto University Faculty of Medicine, 医学部, 講師 (00176512)
Project Period (FY) 1989
KeywordsNicotinic acetylcholine receptor / Sodium channel / Calcium channel / Site-directed mutagenesis / cDNA expression / Patch-clamp / Voltage sensor / Tetrodotoxin
Research Abstract

Neurotransmitter receptors and ionic channels are membrane proteins essential for neural signalling. Cloning and sequencing of cDNAs have revealed the primary structures of different families of receptors and channels. The structural basis for the function of neurotransmitter receptors and ionic channels has been investigated by the combined use of molecular-biological and electrophysiological methods. Thus receptor and channel proteins and their mutants have been produced by expression of the respective cDNAs, and the functional properties of the wild-type and mutant proteins have been analysed. The main findings obtained concern the identification of a channel-forming region of the nicotinic acetylcholine receptor, the identification of a voltage sensor segment responsible for activation of the sodium channel and the functional role of the skeletal muscle slow calcium channel in excitation-contraction coupling.

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Takeshima,H.et al.: "Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor" Nature. 339. 439-445 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Stuhmer,W.et al.: "Structural parts involved in activation and inactivation of the sodium channel" Nature. 339. 597-603 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Mikami,A.et al.: "Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel" Nature. 340. 230-233 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beam,K.G.,Tanabe,T.& Numa,S.: "Structure,function,and regulation of the skeletal muscle dihydropyridine receptor" Ann.N.Y.Acad.Sci.560. 127-137 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Beckh,S.et al.: "Differential regulation of three sodium channel messenger RNAs in the rat central nervous system development" EMBO J.8. 3611-3616 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kaupp,U.B.et al.: "Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclic GMP-gated channel" Nature. 342. 762-766 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yokoyama,S.et al.: "Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells:Primary structure and functional expression from cDNAs" FEBS Lett.259. 37-42 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noda,M.et al.: "A single point mutaion confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II" FEBS Lett.259. 213-216 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Penner,R.et al.: "Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA" FEBS Lett.259. 217-221 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fukuda,K.et al.: "Selective effector coupling of muscarinic acetylcholine receptor subtypes" Trends Pharmac.Sci.Suppl.10. 4-10 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeshima, H., Nishimura, S., Matsumoto, T., Ishida, H., Kangawa, K., Minamino, N., Matsuo, H., Ueda, M., Hanaoka, M., Hirose, T. & Numa, S.: "Primary structure and expression from complementary DNA of skeletal muscle ryanodine receptor" Nature. 339. 439-445 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Stuhmer, W., Conti, F., Suzuki, H., Wang, X., Noda, M., Yahagi, N., Kubo, H. & Numa, S.: "Structural parts involved in activation and inactivation of the sodium channel" Nature. 339. 597-603 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Mikami, A., Imoto, K., Tanabe, T., Niidome, T., Mori, Y., Takeshima, H., Narumiya, S. & Numa, S.: "Primary structure and functional expression of the cardiac dihydropyridine-sensitive calcium channel" Nature. 340. 230-233 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beam, K. G., Tanabe, T. & Numa, S.: "Structure, function, and regulation of the skeletal muscle dihydropyridine receptor" Ann. N. Y. Acad. Sci.560. 127-137 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Beckh, S., Noda, M., Lubbert, H. & Numa, S.: "Differential regulation of three sodium channel messenger RNAs in the rat central nervous system during development" EMBO J.8. 3611-3616 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kaupp, U. B., Niidome, T., Tanabe, T., Terada, S., Bonigk, W., Stuhmer, W., Cook, N. J., Kangawa, K., Matsuo, H., Hirose, T., Miyata, T. & Numa, S.: "Primary structure and functional expression from complementary DNA of the rod photoreceptor cyclinc GMP-gated channel" Nature. 342. 762-766 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yokoyama, S., Imoto, K., Kawamura, T., Higashida, H., Iwabe, N., Miyata, T. & Numa, S.: "Potassium channels from NG108-15 neuroblastoma-glioma hybrid cells: Primary structure and functional expression from cDNAs" FEBS Lett.259. 37-42 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noda, M., Suzuki, H., Numa, S. & Stuhmer, W.: "A single point mutaion confers tetrodotoxin and saxitoxin insensitivity on the sodium channel II" FEBS Lett.259. 213-216 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Penner, R., Neher, E., Takeshima, H., Nishimura, S. & Numa, S.: "Functional expression of the calcium release channel from skeletal muscle ryanodine receptor cDNA" FEBS Lett.259. 217-221 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fukuda, K., Kubo, T., Maeda, A., Akiba, I., Bujo, H., Nakai, J., Mishina, M., Higashida, H., Neher, E., Marty, A. & Numa, S.: "Selective effector coupling of muscarinic acetylcholine receptor subtypes" Trends Pharmac. Sci. Suppl.10. 4-10 (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-26  

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