1990 Fiscal Year Final Research Report Summary
Chemical Reaction of Polysaccharides and Their Applications to Biomedical Materials
| Project/Area Number |
01045007
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| Research Category |
Grant-in-Aid for international Scientific Research
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| Allocation Type | Single-year Grants |
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| Section | University-to-University Cooperative Research |
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| Research Institution | Gunma University |
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Principal Investigator |
NAKAMURA Yoshio Professor, Faculty of Technology, Gunma University, 工学部, 教授 (90008425)
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| Co-Investigator(Kenkyū-buntansha) |
PHILLIPS Glyn O. President, The North East Wales Institute, United Kingdom, 学長
KOBAYASHI Norio Lecturer, Medicine Faculty, Gunma University, 医学部, 講師 (60008539)
KOMOTO Tadashi Assistant Professor, Faculty of Technology, Gunma University, 工学部, 助教授 (00016643)
ARAI Kozo Professor, Faculty of Technology, Gunma University, 工学部, 教授 (60008456)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Heparin / Anticoagulant activity / 1-Fluoro-2, 4-dinitrobenzene / 2, 4, 6-Trinitrobenzene Sulfonic Acid / Hyaluronic Acid / Silk Fibroin Membrane / Egg Shell Membrane / Biocompatibility |
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| Research Abstract |
1. It has been reported that the anticoagulant activity of heparin depends on the molecular weight and the content of sulfate group in heparin, but the causes have not yet elucidated sufficiently. Therefore, in order to clarify influence of the chemical structure on the anticoagulant activity of heparin, chemical modification of heparin was carried out and its anticoagulant activity was studied. Heparin was dinitrophenylated (DNP) with 1-fluoro-2, 4-dinitrobenzene. DNP-Amino and DNP-hydroxyl groups were detected, and the anticoagulant activity of DNP-heparin was greatly decreased. Heparin was also reacted with 2, 4, 6-trinitrobenzene sulfonic acid (TNBS), and it was observed that the reactivities of TNBS with amino group from alpha-amino acid and hexosamine residue were greatly different.
2. Hyaluronic acid is carbohydrate of high molecular weight with remarkable water retention and with function preventing invasion of foreign materials to tissue. The purpose of this study is to develop an excellent covering materials for seriously burnt skin and an artificial skin by immobilisation of hyaluronic acid onto proteins with superior biocompatibility. Hyaluronic acid was immobilised onto silk fibroin by cyanuric chloride. The immobilised samples contained much more water than silk fibroin. Hyaluronic acid was also immobilised onto egg shell and gelatin membranes by formaldehyde. The immobilised membranes showed the increase of water content with increasing immobilised hyaluronic acid and an excellent biocompatibility without changing the permeability of oxygen being soluble in water as compared to original membranes.
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