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1991 Fiscal Year Final Research Report Summary

A study of the molecular genetic of virulence of fish and domestic animal-pathogens

Research Project

Project/Area Number 01045027
Research Category

Grant-in-Aid for international Scientific Research

Allocation TypeSingle-year Grants
SectionUniversity-to-University Cooperative Research
Research InstitutionMiyazaki University

Principal Investigator

KITAO Tadatoshi  Professor, Faculty of Agriculture, Miyazaki University, 農学部, 教授 (60094075)

Co-Investigator(Kenkyū-buntansha) ACHARIYA Sai  チュラロンコン大学, 獣医学部, 助教授
KRIENSAG Poo  チュラロンコン大学, 獣医学部, 助教授
WATANA Watan  チュラロンコン大学, 獣医学部, 助教授
NIKOM Chaise  チュラロンコン大学, 獣医学部, 助教授
SOMATINAT Wo  チュラロンコン大学, 獣医学部, 助教授
BOONMEE Suny  チュラロンコン大学, 獣医学部, 助教授
TED Tesaprat  チュラロンコン大学, 獣医学部, 助教授
KRIENSAG Sai  チュラロンコン大学, 獣医学部, 助教授
SONGKRAM Lun  チュラロンコン大学, 獣医学部, 助教授
YOSHIDA Terutoyo  Assistant Professor, Faculty of Agriculture, Miyazaki University, 農学部, 助手 (20240294)
ENDO Makoto  Associate Professor, Faculty of Agriculture, Miyazaki University, 農学部, 助教授 (80128355)
SUIKO Masahito  Associate Professor, Faculty of Agriculture, Miyazaki University, 農学部, 助教授 (00128357)
AOKI Takashi  Professor, Faculty of Agriculture, Miyazaki University, 農学部, 教授 (00051805)
KONDO Fusao  Professor, Faculty of Agriculture, Miyazaki University, 農学部, 教授 (40145404)
TATEYAMA Susumu  Professor, Faculty of Agriculture, Miyazaki University, 農学部, 教授 (90041003)
SUNYASOOTCHAREE Boonmee  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
TESAPRATEEP Ted  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
SAITANU Kriengsag  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
LUANGTONGKUM Songkram  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
WONGSAWANG Somatinat  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
POONSUK Kriengsag  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
WATANAVIGAN Watana  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
SAILASUTA Achariya  Assistant Professor, Faculty of Veterinary Science, Chulalongkorn University
CHAISERI Nikom  Associate Professor, Faculty of Veterinary Science, Chulalongkorn University
Project Period (FY) 1989 – 1991
KeywordsVirulence factor / Fish-pathogenic bacteria / Domestic animal pathogenic bacteria / Protooncogene / Aujeszky's disease / Immune system / Protective mechanism / drug residues / Tyrosin-O-sulfate / Prawn disease / hemolysin / Molecular cloning
Research Abstract

The main research project is divided into seven sub-projects.
1) Studies on protooncogenes of genomic DNA in domestic animals in Japan and Thailand.
Protooncogenes, c-myc, c-ros-1, c-erbB, c-yes-1, v-myc, v-Ki-ras, v-HA-ras, of genomic DNA in water buffaloes, ducks, chicken, dogs and cats in Thailand has been studied and compared with that of Japanese. The genetic data of each animals were recorded and it was found that these oncogenes were well preserved in every animals studied.
2) Immunohistochemical demonstration of Aujeszky's antigen in tissue of Thai pig.
Diagnostic evidence of Aujeszxy's disease from hog cholera has been obtained by immunohistochemical study of distribution of antigen of Aujeszky's disease in pig in Thailand.
3) Immune system and protective mechanism against fish-pathogenic bacteria in fish.
This study demonstrated that immunoactive peptide, chicken fermented products had the ability to stimulate phagocytic chemiluminescent responses of rainbow trout, and induce non-s … More pecific protection. It also confirmed that fish phagocytes play an important role in non-specific defense mechanisms against pathogens.
4) Detection and identification of antibacterial agents residues in animal tissues.
Using samples positive for antibacterial agent residue in microbiological screening tests (bioassay) of meat collected from open markets in Bangkok, Thailand, identification tests were carried out by both TLC-bioautographic and HPLC methods. Residual oxytetracycline obviously demonstrated in extracts from swine muscle (3), kidney (15) and liver, and extracts tissues and oxolinic acid from 10 samples of black tiger prawn by HPLC analysis.
5) Tyrosine-O-sulfate of proteins in oncogenesis.
The project was aimed at the elucidation of the relevance of tyrosine sulfation of proteins in the oncogenesis, and that on the tertiary structure of the sulfated proteins. We demonstrated that the free tyrosine sulfate excretion decreased viral infection and oncogenic transformation of the cells. Modification of recombinant proteins by tyrosine sulfate could be a vital process for the biological activity of some proteins depending on the final tertiary structure.
6) Pathological aspects of prawn and fish culture in Thailand.
Samples of some prawn disease, and hemocytic enteritis, were collected in Thailand 1989 to 1991. They were examined to reveal their pathogen and pathological appearance.
7) Molecular cloning and expression in Escherichia coli of the structure gene for the hemolysin aeromonads.
Five hemolysin genes(AHH-1 - AHH-5) were cloned into a plasmid vector in E. coli from Aeromonas hydrophila. There were no homology of the deduced amino acid sequences in AHH-1, AHH-2 and aerolysin (Howard & Buckley 1986). The deduced amino acid sequences of AHH-3, AHH-4, AHH-5 and aerolysin showed a significant degree of sequence homology each other. Three different extracellular hemolysin genes were cloned from A. salmonicida. Three different hemolysin genes were also cloned from Vibrio anguillarum. The hemolysin genes having different structures occur in A. hydrophila, A. salmonicida and V. anguillarum isolates from different geographical locations. Less

  • Research Products

    (42 results)

All Other

All Publications (42 results)

  • [Publications] Terutoyo Yoshida: "Spontaneous rosette formation of carp peripheral leucocytes with mammalian red blood cells." Nippon Suisan Gakkaishi. 56. 1579-1585 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terutoyo Yoshida: "The opsonic effect of specific immune serum on the phagocytic and chemiluminescent response in rainbow trout,Oncorhynchus mykiss phagocytes." Fish Pathology. 26. 29-33 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tadatoshi Kitao: "Analysis of protective mechanisms in cultured ayu,Plecoglossus altivelis Temminck and Schlegel,administered Vibrio vaccine by the immersion method." Journal of Fish Diseases. 14. 375-381 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Magdy Khlil Soliman: "First record of association of Aeromonas hydrophila with mortalities of cultured mullet,Mugil capito in Egypt." Fisheries Sciences Journal(Thailand). 2. 90-95 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terutoyo Yoshida: "Immunomodulatory effects of the fermented products of chicken egg,EF203 on raibow trout,Oncorhynchus mykiss." Aquaculture.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ted Tesaprateep: "Preliminary Survey on protoncogenes in normal Thai domestic animals" Proceedings of the 7th FAVA Congress. 325-332 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriaki Miyoshi: "Protoーoncogenes of genomic DNA in clinically normal animals of various species." Am.J.Vet.Res.52. 940-944 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yuko Mutoh: "Retrospective study of aujeszxy's disease in pig in THAILAND using immunohistochemical method." Thai J.Vet.Med.20. 553-567 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriaki Miyoshi: "Abnormal struture of the canine oncogene,related to the human cーyesー1 oncogene,in canine mammary tumor tissue." Am.J.Vet.Res.52. 2046-2049 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Boonmee Sunyasoocharee: "Application of the immunohistochemical method for detection of aujeszky's disease virus infection in the field cases of hog cholera in Thailand." Thai J.V.Med.(1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noriaki Miyoshi: "Amplification of the canine oncogene,related to the human cーyesー1 oncogene,in canine lymphoid neoplastic tissue." Am.J.Vet.Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fusao Kondo: "Continious simple and rapid method for the detection,extraction and identification of residual antibacterial agents in meat using an agar block from the inhibition zone on minimum medium." J.Food Protection.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fusao Kondo: "Determination of pyridonecarboxylic acids by reverseーphase highーperformance liquid chromatography." J.Food Protection.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Fusao Kondo: "Method for determination of nicarbazin residues in eggs by reverseーphase highーperformance liquid chromatography." J.Food Protection.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Aoki: "Clonling and characterization of the haemolysin determinants from Aeromonas hydrophila." J.Fish Diseases. 14. 303-312 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ikuo Hirono: "Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila." Miribial Pathogensis. 11. 189-197 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takashi Aoki: "Cloning of hemolysin genes of aeromonads" Proceedings of the 19th USーJapan Meeting on Aquaculture.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masahito Suiko: "Quantitative determination of tyrosineーOーsulfate in urin: Application of ionーpair reverseーphase high performance liquid chromatography in the analysis of tyrosineーOーsulfate." Clinica.Chimica.Acta. 193. 193-198 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] MingーChen Liu: "Identification of complex between the tyrosineーOーsulfateーbinding protein and tyrosineーsulfated proteins in bovine liver membrane lysate." Biochemical Journal. 275. 259-262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Jian Rong Han: "Postーtranslational modification and binding properties of the apically secreted 80 KDa glycoprotein from madin derby canine kidney cells." Archives of Biochemistry and Biophysics. 286. 337-345 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ted Tesaprateep: "Histopathology of Vibrio alginolyticus infection in Penaeus monodon" Thai J.Vet.Med.20. 538-550 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Terutoyo Yoshida: "Spontaneous rosette formation of carp peripheral leucocytes with mammalian red blood cells." Nippon Suisan Gakkaishi. 56. 1579-1585 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terutoyo Yoshida: "The opsonic effect of specific immune serum on the phagaocytic and chemiluminescent response in rainbow trout, Oncorhynchus mykiss phagocytes." Fish Pathology. 26. 29-33 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tadatoshi Kitao: "Analysis of protective mechanisms in cultured ayu, Plecoglossus altivelis Temminck and Schlegel, administered Vibrio vaccine by the immersion method." Journal of Fish Diseases. 14. 375-381 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Magdy khlil Soliman: "First record of association of Aeromonas hydrophila with mortalities of cultured mullet, Mugil capito in Egypt." Fisheries Sciences Journal (Thailand). 2. 90-95 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Terutoyo Yoshida: "Immunomodulatory effects of the fermented products of chicken egg. EF203 on rainbow trout, Oncorhynchus mykiss" Aguaculture.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriaki Miyoshi: "Amplification of the canine oncogene, related to the human c-yes-1 oncogene, in canine lymphoid neoplastic tissue." Am. J. Vet. Res.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fusao Kondo: "Continious simple and rapid method for the detection, extraction and identification of residual antibacterial agents in meat using aniagaar block from the inhibition zone on minimum medium." J. Food Protection.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fusao Kondo: "Determination of pyridonecarboxylic acids by reverse-phase high-performance liquid chromatography." J. Food Protection.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Fusao Kondo: "Method for determination of nicarbazin residues in eggs by reverse-phase high performance liquid chromatography." J. Food Protection.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Aoki: "Cloning and characterization of the haemolysin determinants from Aeromonas hydrophila." J. Fish Diseases. 14. 303-312 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ted tesaprateep: "Preliminary Survey on protooncogenes in normal Thai domestic animals." Proceedings of the 7th FAVA Congress.325-332 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriaki Miyoshi: "Proto-oncogenes of genomic DNA in clinically normal animals of various species." Am. J. Vet. Res.52. 940-944 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yuko Mutoh: "Retrospective study of aujeszxy's disease in pig in THAILAND using immunohistochemical method." Thai J. Vet. Med.20. 553-567 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriaki Miyoshi: "Abnormal structure of the canine oncogene, related to the human c-yes-1 oncogene, in canine mammary tumor tissue." Am. J. Vet. Res.52. 2046-2049 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Boonmee Sunyasoocharee: "Application of the immunohistochemical method for detection of aujeszky's disease virus infection in the field cases of hog cholera in Thailand." Thai J. Vet. Med.(1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ikuo Hirono: "Nucleotide sequence and expression of an extracellular hemolysin gene of Aeromonas hydrophila" Miribial Pathogensis. 11. 189-197 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Aoki: "Cloning of hemolysin genes of aeromonads" Proceedings of the 19th US-Japan Meeting on Aquaculture.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masahito Suiko: "Quantitative determination of tyrosine-O-sulfate in urin : Application of iron-pair reverse-phase high performance liquid chromatography in the analysis of tyrosine-O-sulfate." Clinica. Chimica Acta. 193. 193-198 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ming-Chen Liu: "Identification of complex between the tyrosine-O-sulfate-binding protein and tyrosine-sulfated proteins in bovine liver membrane lysate." Biochemical Journal. 275. 259-262 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Jian Rong Han: "Post-translational modification and binding properties of the apically secreted 80 KDa glycoprotein from madin derby canine kidney cells." Archives of Biochemistry and Biophysics. 286. 337-345 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ted Tesaprateep: "Histopathology of Vibrio alginolyticus infection in Penaeus monodon" Thai J. Vet. Med.20. 538-550 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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