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1990 Fiscal Year Final Research Report Summary

The Construction of Halo-Tolerant Higher Plants which were Transformed by Marine Alga Na^+-ATPase gene.

Research Project

Project/Area Number 01400001
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field 広領域
Research InstitutionTsukuba University

Principal Investigator

FUJII Tadashi  University of Tsukuba Institute of Biological Sciences Professor, 生物科学系, 教授 (20011611)

Co-Investigator(Kenkyū-buntansha) HARA Yukichi  東京医科歯科大, 医学部, 助教授 (00092429)
SATOH Shinobu  University of Tsukuba Institute of Biological Sciences Assistant Professor, 生物科学系, 講師 (70196236)
Project Period (FY) 1989 – 1990
KeywordsRaphidophyceae / Na^+-ATPase / halo-tolerant
Research Abstract

The ability which can export intracellular sodium ions can be transformed usual higher plants to halotolerant. The Na^+ K^+-ATPase of animal cells or Na^+-ATPase of raphidophycean H. akashiwo was able to transport Na ions. The Na^+-ATPase of H. akasiwo was consequently proper enzyme for the transformation. A DNA between conserved parts of eucaryotic ATPase was amplified from H. akasiwo cDNA library with PCR method. The Na^+-ATPase of H. akashiwo was cloned with the amplified DNA as a probe. Two kinds of ATPase molecules was cloned consequently. The amino acid sequences and the secondary structures analysis showed the homology to other species' ATPases(70% : protozoan L. donovani, 40-50% : higer plants and fungi, 20-30% : higher animals). This suggests that the ATPase amino acid sequences tend to conserve among systematical close to species rather than the functional characters (ion selectivity, etc). Rescreeing was needed to the reason why the two cDNA clones were short of complete coding regions. The most expressing cell period of the genes was determined with northern hybridization and 5' end probe was successed to get the enough length cDNA (3.4kb).

  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] 和田 雅人・佐藤 忍・笠毛 邦宏・藤伊 正: "Presence of a Na^+ーactivated ATPase in the Plasma Membrane of the Maline Raphidophycean Heterosigma akashiwo" Plant Cell Physiolgy. 30(6). 923-928 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 和田 雅人・佐藤 忍・浦山 修・原 諭吉・井川 洋二・藤伊 正: "Immunological homology between Na^+ーactivated ATPase from marine alga Heterosigma akashiwo and animal Na^+,K^+ーATPase." Biochemical and Biophysical Research Communications.

    • Description
      「研究成果報告書概要(和文)」より

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Published: 1993-08-12  

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