1992 Fiscal Year Final Research Report Summary
Functional analysis of chloroplast genes and its utilization to breeding
| Project/Area Number |
01440006
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Breeding science
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| Research Institution | Nagoya University |
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Principal Investigator |
FUTSUHARA Yuzo Nagoya U., Fac. Agric., Professor, 農学部, 教授 (70023405)
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| Co-Investigator(Kenkyū-buntansha) |
HATTORI Kazumi Nagoya U., Fac. Agric., Assoc. Professor, 農学部, 助教授 (40023494)
TEZUKA Takafumi Nagoya U., Fac. Agric., Assoc. Professor, 農学部, 助教授 (10109316)
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| Project Period (FY) |
1989 – 1992
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| Keywords | Rice / Nicotiana plant / Cell fusion / Protoplast culture / Chloroplast / Gene transferring |
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| Research Abstract |
1. When the structures of chloroplast DNA in rice in each genome were examined, it was confirmed that these variations were base substitutions and deletions of 100 base units. It was demonstrated also that deletions were induced by recombinations with the direct repeat of about 10 base. Moreover, when the chloroplast DNAs of the genus Oryza plant with BBCC genome were examined, chloroplast DNA of BB genome and CC genome were discovered.2. For examining the chloroplast genome, the plant which has same unclear but different chloroplast in necessary. It is found that the combination of Nicotiana glauca and N. langsdorffii is the most suitable for the experiment. Each chloroplast of N. langsdorffii or N. glauca contained two types of calli. 3. To produce similar somatic hybrids using cell fusion in rice, the protoplast culture was done. Protoplasts could grow until cell masses stage, but plantlets could not be produced. 4. When various chlorophyll mutants were analyzed by using simple analytical procedure for thylakoid membrane, it was shown that some of these chlorophyll mutants express variation in thylakoid. 5. Some genes were directly transferred to some plant tissues by using Laser Cell Processor. It was found that the gene expresses in the plantlet regenerated directly (not via callus). But whether the gene was transferred to the chloroplast is still not known yet.
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[Publications] Hiratsuka,J., H.Shimada, R.Whittier, T.Ishibashi, M.Sadamoto, M.Mori, C.Kondo, Y.Honji, C.Sun, B.Meng, Y.Li, A.Kanno, Y.Nishizawa, A.Hirai, K.Shinozaki and M.Sugiura: "The complete sequence of the rice (Oryza sativa) chloroplast genome; Intermolecular recombination between distinct tRNA genes accounts for a major plastid DNA inversion during the evolution of the cereals." Mol.Gen.Genet. 217. 185-194 (1989)
Description
「研究成果報告書概要(欧文)」より
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