• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1992 Fiscal Year Final Research Report Summary

Study of synaptic transmissions on hair cells

Research Project

Project/Area Number 01440021
Research Category

Grant-in-Aid for General Scientific Research (A)

Allocation TypeSingle-year Grants
Research Field Neurophysiology and muscle physiology
Research InstitutionKYOTO UNIVERSITY (1992)
Okazaki National Research Institutes (1989-1991)

Principal Investigator

OHMORI Harunori  Fact.Med.Dept.Physiol. Professor, 医学部, 教授 (30126015)

Project Period (FY) 1989 – 1992
KeywordsHair cell / afferent synapse / efferent synapse
Research Abstract

Hair cells of the inner ear is the transducer cell of mechanical energy. Namely, the informations of sound, balance and accelerations are transduced into the electrical signal and then transmitted to the central nervous system. From the central nervous system some feedback informations are returned to the peripheral hearing organ to inhibit the hair cell. I have aimed in this research to investigate both the afferent and efferent synapses on to the hair cell, by utilizing a slice preparation of the cochlear. However, making a good slice of the hearing organ was extremely difficult, because of the mixture of hard and soft structures. I have therefore focused on the analysis by utilizing cutout preparation of vestibular organ and on the experiments with isolated hair cells. Afferent synapse activities were recorded in the cut-out preparation of vestibular organ, and the differences of the synaptic potential appearance, probably the mechanism of synapse transmission too, were detected between bouton type and calyx type synapses. The isolated hair cells were mainly studied in the analysis of acetylcholine effects. Acetylcholine hyperpolarized the hair cell membrane by increasing intracellular Ca concentration and by activating K conductance. The transmitter released from hair cell was concluded most likely as glutamate also by utilizing the isolated hair cell preparation together with cultured neuron with high glutamate receptor sensityvity.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] Shigemoto,T.: "Muscarinic agonists and ATP increase the intracellular Ca concentration in chick cochlear hair cells." Journal of Physiology. 420. 127-148 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamaguchi,K.: "Voltage-gated and chemically-gated ionic channels in the cultured cochlear ganglion neurone of the chick." Journal of Physiology. 420. 185-206 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ashmore,J.F.: "Control of intracellular calcuim by ATP in isolated outer hair cells of the guinea pig cochlea." Journal of Physiology. 428. 109-131 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yamashita,M.: "Synaptic responses to mechanical stimulation in calyceal and bouton type vestibular afferents in isolated semicircular canal ampullae of chicken." Experimental Brain Research. 80. 475-488 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigemoto,T.: "Muscarinic receptor hyperpolarizes cochlear hair cells of chick by activating Ca activated K channels." Journal of Physiology. 442. 669-690 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ohmori,H.: "Ion channels for the mechano-electrical transduction and efferent synapse of the hair cell." Advances of Biophysics. 28. 1-30 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shigemoto,T: "Muscarinic agonists and ATP increase the intracellular Ca concentration in chick cochlear hair cells." Journal of Physiology. 420. 127-148 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamaguchi,K.: "Voltage-gated and chemically-gated ionic channels in the cultured cochlear ganglion neurone of the chick." Journal of Physiology. 420. 185-206 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ashmore,J.F.: "Control of intracellular calcium by ATP in isolated outer hair cells of the guinea pig cochlea." Journal of Physiology. 428. 109-131 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yamashita,M.: "Synaptic responses to mechanical stimulation in calyceal and bouton type vestibular afferents studied in an isolated preparation of semicircular canal ampullae of chicken." Experimental Brain Research. 80. 475-488 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shigemoto,T.: "Muscarinic receptor hyperpolarizes cochlear hair cells of chick by activating Ca activated K channels." Journal of Physiology. 442. 669-690 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ohmori,H.: "Ion channels for the mechano-electrical trnasduction and efferent synapse of the hair cell." Advances in Biophysics. 28. 1-30 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kimituki,T.: "The effect of caged calcium release on the adaptation of the transduction current in chick hair cells." Journal of Physiology. 458. 27-40 (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1994-03-24  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi