1990 Fiscal Year Final Research Report Summary
Molecular Identification and Immunological Significance of I-J Molecule.
| Project/Area Number |
01440033
|
|---|
| Research Category |
Grant-in-Aid for General Scientific Research (A)
|
| Allocation Type | Single-year Grants |
|---|
| Research Field |
Immunology
|
|---|
| Research Institution | University of Tokyo |
|---|
Principal Investigator |
TADA Tomio University of Tokyo, professor, 医学部・医学科, 教授 (10009136)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SEIKI Makoto University of Tokyo, Associate Professor, 医学部・医学科, 助手 (50226619)
SANO Kunio University of Tokyo, Associate Professor, 医学部・医学科, 助手 (20192601)
KARASUYAMA Hajime University of Tokyo, Associate Professor, 医学部・医学科, 助手 (60195013)
|
|---|
| Project Period (FY) |
1989 – 1990
|
| Keywords | I-J / Major histocompatibility complex / Suppressor T cell / Helper T cell / Ca^<2+> influx / Signal transduction |
|---|
| Research Abstract |
I-J has been an enigmatic molecule on T cells which undergoes a systematic somatic alteration according to the environmental major histocompatibility complex (MHC) during their ontogeny of T cells as demonstrated in radiation bone marrow chimeras and class II gene transgenic mice. Thus, I-J is not a direct product of an MHC gene as originally thought, but is an adaptive molecule to a self MHC polymorphism most likely to be a receptor-like molecule for self. In order to study the molecular nature of I-J, we have established a series of IL-2 dependent T cell clones from different haplotype origins including radiation bone marrow chimeras. They are either helper (Th) or suppressor (Ts) clones with different MHC restriction specificities. They are mostly CD4^+ Th and Ts clones except for a few CD8^+ Ts clones. Ts subtype was defined by its inability to produce both IL-2 and IL-4, lack of helper activity for B cells, and by its strong inhibitory activity for the antibody response of MHC-mat
… More
ched T and B cells.
Many of the H-2^k-restricted clones were found to be positive for the staining with a monoclonal anti-I-J^K. Both I-A^k- and I-E^k- restricted clones derived from different origins including H-2^b- > H-2^k chimeras expressed the same I-J^k epitope. The I-J molecule was not co-modulated with TcR/CD3 complex.
By immunoprecipitation and subsequent one- or two-dimensional gel analyses of the lysate of these T cell clones, I-J molecule was found to be a novel dimeric surface molecule of MW 86,000 composed of 43,000 glycopeptide subunits differing from TcR heterodimer, MHC class II antigen, and previously known dimeric proteins of T cells. A monomeric form also existed on some T cell clones. Protein sequencing and gene cloning approaches are now under way.
The I-J polypeptide seems to play an important role in the regulation of early signal transduction in T cells after antigen-recognition. When T cell clones with the I-J^k epitope was pretreated with the monoclonal anti-I-J^k, the Ca^<++> influx induced by a subsequent stimulation with antigen-pulsed antigen-presenting cell was greatly inhibited. The Ca^<++> response induced by anti-TcR heterodimer but not by anti-CD3 or Con A was similarly inhibited by the anti-IーJ. The ligation of I-J molecules by intact antibody but not by Fab was required for the initiation of suppression. The pattern of inhibition of Ca^<++> influx was very similar to that observed in Th clones preincubated with Ts clones. These results indicate that the negative signal from I-J receptor may inhibit the expansion of self MHC-reactive T cells both in ontogeny and in periphery. Less
|
