1990 Fiscal Year Final Research Report Summary
Immunogenetical Nature of the Primary Malignant Lymphoma of the Brain : Analyses of Immunoglobulin Gene Rearrangements.
| Project/Area Number |
01440059
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| Research Category |
Grant-in-Aid for General Scientific Research (A)
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| Allocation Type | Single-year Grants |
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| Research Field |
Cerebral neurosurgery
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| Research Institution | Niigata University |
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Principal Investigator |
KUMANISHI Toshiro Niigata University, Brain Research Institute, Professor, 脳研究所, 教授 (40018601)
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| Co-Investigator(Kenkyū-buntansha) |
USUI Hiroshi Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (20192510)
ABE Satoshi Niigata University, Brain Research Institute, Assistant, 脳研究所, 助手 (90202663)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Malignant Lymphoma / Brain Tumor / Non Hodgkin Lymphoma / Immunoglobulin / Gene Rearrangement |
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| Research Abstract |
Using the Southern blot hybridization technique, seven cases of the primary malignant lymphomas of the brain, histologically diffuse large cell lymphoma, were examined for the immunoglobulin gene rearrangements. In these lymphoma cases, control germーline DNAs of peripheral leukocytes or of nonneoplastic tissues from the same patients were available and were examined in parallel. In six lymphomas, the rearrangements were observed in both heavy and light chain genes, providing a strong evidence for a B cell lineage of these tumors. On the other hand, in the remaining lymphoma, the rearrangement was observed only in the heavy chain genes. Despite these, immunohistochemical examination revealed positive stainings for heavy and light chain immunoglobulins in tumor cells, suggesting the occurrence of light chain gene rearrangements at the undetectable level. The patterns of the rearrangements in all the other lymphomas were comparable to the immunohistochemical findings. Thus, B lymphocytic differentiation at the gene level was demonstrated in all of the primary intracerebral malignant lymphomas examined. Since no more than two rearrangements were detected in each immunoglobulin gene, these lymphomas were considered to be monoclonal in nature.
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