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1991 Fiscal Year Final Research Report Summary

Development of High Quarity of Chemical Sensors Based on Polymeric Thin Film Coating

Research Project

Project/Area Number 01470064
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 工業分析化学
Research InstitutionTokyo University of Agriculture and Technology

Principal Investigator

OYAMA Noboru  Tokyo University of Agriculture and Technology, Department of Applied Chemistry, Professor, 工学部, 教授 (40134845)

Project Period (FY) 1989 – 1991
KeywordsFilm coated electrode / Amperometric sensor / Glucose / Cholesterol / Flow injection analysis / Quartz crystal oscillator / Admittance spectra / Affinity sensing
Research Abstract

Polymer coated electrodes (PCEs), which consist of a metallic or carbon conductor direct coated with an ion-sensitive, electrocatalytic, or enzyme membrane, are very attractive potentiometric and amperometric sensors due to their simplicity of operation, superior amperometric response characteristics, capability of minimization, etc., compared with conventional membrane electrodes.
In this research, new amperometric sensors for determination of glucose and cholesterol based on bilayer film coating have been fabricated and their sensor characteristics under a steady-state condition have been examined. The glucose or cholesterol concentration could be monitored by measuring the current for H_2O_2 or O_2 reduction electrocatalyzed inner film where H_2O_2 was produced orO_2. was consumed by the enzyme reaction in the outer enzyme films. The reproducible relationship between glucose or cholesterol concentration and sensor output was obtained. The PCEs functioned as an electrochemical detecto … More r for flow injection analysis. Substantial unprovement in the selectivity of arnperometric detection for flow injection system was observed as a result of an electrostatic interaction between the positively charged electropolymerized film and analysis, that is, cationic species (e. g., dopamine, 1-norepinephrine and DL-3, 4-dihydroxyphenylalanine) exhibited little response, while the response of neutral and anionic species (e. g., 3, 4-dihydroxyphenylacetic acid, 4-hydroxy-3-methoxyphenylacetic acid and catechol) was only slightly changed at the polymer coated electrode compared with the bare electrode.
The complex admittance measurements of the quartz crystal oscillators with and without a film coating, which are in contact with air, moisture and supporting electrolyte aqueous media of various concentrations have been carried out over a frequency range covering the area of the resonant frequency of each oscillator system, and the results have been represented in the form of conductance and susceptance spectra (i. e., conductance vs. frequency plots and susceptance vs. frequency plots). According to the electrical equivalent circuit model conventionally proposed for a piezoelectric quartz crystal, the related circuit parameters (resistance R, inductance L, capacitance C and static capacitance C_0) have been evaluated, especially as a function of the concentration of supporting electrolyte. The change of electrolyte concentration-dependent swelling of the film was characterized by the evaluated parameters. It is demostrated that the method described can can apply the characterized kinematic viscosity and affinity sensing of the organic, inorganic and biological thin films. The quartz crystal microbalance (QCM) when operated in liquids was shown to retain its high mass sensitivity. The QCM has therefore been applied to the mass changes occurring during redox in thin polymer films. Solvent transport during electroneutrality requiring ion transport has been qualitatively demonstrated in the case of electrodes modified with thin film of poly (vinyferrocene), polyaniline, etc. Less

  • Research Products

    (20 results)

All Other

All Publications (20 results)

  • [Publications] Noboru Oyama: "A Redox-Active Polymer Film Mediated Enzyme Electrode for Amperometric Determination of Free Cholesterol." Electroanal.3. 655-671 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeo Ohsaka: "Electrocatalysis of Poly(2,3-diamininaphthalene)-filmed Electrodes for Reduction of Oxyger_4" J.Chem.Soc.Chem.Commun.7. 487-488 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.J.Kelly: "Determination of Ion and Solvent Transport in an Osmium Polymer Film using a Quartz Crystal Microbalance." J.Phys.Chem.95(23). 9579-9584 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noboru Oyama: ""in-situ" Quartz Crystal Microbalance Responses and Electrochemistry Regarding LB Film of Viologen Polyion Complexes on Electrode Surfaces." J.Electroanal.Chem.(1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Takeo Ohsaka: "Electrocatalysis of O_2 Reduction at Poly(o-phenylenediamine) and Poly(o-aminophenol)-filmed Glassy Carbon Electrodes." J.Chem.Soc.Chem.Commun.16. 1072-1073 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.J.Kelly: "電気化学マイクロバランス法による電解重合ポリピロ-ル膜におけるイオンの動的挙動のin situ解析" 分析化学. 40. 835-841 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noboru Oyama: ""Voltammetric Diagnosis of Charge Transport on Polymer Coated Electrodes."" John Wiley, 333-402 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 小山 昇: "「高分子錯体ー動的相互作用と電子過程」ー第4編4章、パルス法による電荷移動反応の観測ー" 学会出版センタ-, 243-262 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Noboru Oyama: "A Solid-State Reference Electrode Based on Bilayer Coating with Poly (p, p, -biphenol) and Polyimide Films for the Gate of Field Effect Transistor." Anal. Sci.5. 729-734 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideharu Daifuku: "A study of the redox reaction mechanisms of polyaniline using a quartz crystal microbalance." J. Electroanal. Chem.274. 313-318 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Ohsaka: "Surface-Modified Electrochemical Detector for Flow Injection Analysis. Poly (N, N-dimethylaniline) -Coated Glassy Carbon Electrodes." Denki Kagaku. 58. 1136-1142 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Andrew J. Kelly: "Determination of in-situ solvent transport by isotopic substitution in an osmium polymer film using a quartz crystal microbalance." J. Electroanal. Chem.287. 185-190 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Ohsaka: "A New Amperometric Glucose Sensor Based on Bilayer Film Coating of Redox-Active Clay Film and Glucose Oxidase Enzyme Film." Bull. Chem. Soc. Jpn. 63. 2646-2652 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noboru Oyama: "Polymer-Complex Mediated Enzyme Electrodes for Amperometric Determination of Glucose." J. Macromol. Sci. -Chem.A27(9-11). 1203-1216 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hideharu Daifuku: "Quartz Crystal Microbalance Study on Redox Reaction Mechanism of Polyaniline." Synthetic Metals. 41-43. 2897-2900 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noboru Oyama: "A Redox-Active Polymer Film Mediated Enzyme Electrode for Amperometric Determination of Free Cholesterol." Electroanalysis. 3. 665-671 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Ohsaka: "Electrocatalysis of Poly (2, 3-diaminonaphthalene)-filmed Electrodes for Redustion of oxygen." J. Chem. Soc. Chem. Commun.487-488 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeo Ohsaka: "Electrocatalysis of O_2 Redustion at Poly (o-phenylenediamine)-and Poly (o-aminophenol)-coated Glassy Carbon Electrodes." J. Chem. Soc. Chem. Commun.1072-1073 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Andrew J. Kelly: "Determination of lon and Solvent Transport in an Osmium Polymer Film Using a Quartz Crystal Microbalance." J. Phys. Chem.,. 95. 9579-9582 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takeyoshi Okajima: "Electrical Equivalent Circuit Parameters for Montmorillonite Clay Film-Coated Quartz Crystal Oscillators in Contact with Air, Moisture and Electrolyte Solutions"

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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