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1990 Fiscal Year Final Research Report Summary

Biopolymer Functions under High Pressure

Research Project

Project/Area Number 01470103
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子物性
Research InstitutionKyoto Institute of Technology (1990)
University of Fukui (1989)

Principal Investigator

KUNUGI Shigeru  Kyoto Institute of Technology, Faculty of Textile Science Professor, 繊維学部, 教授 (70111929)

Co-Investigator(Kenkyū-buntansha) NOMURA Akihiko  Fukui University, Faculty of Engineering, Professor, 工学部, 教授 (10001041)
Project Period (FY) 1989 – 1990
KeywordsHigh pressure / Biopolymers / Proteoses / Nucleases / Chemical Modification / Denaturation / Proteins
Research Abstract

Pressure is a thermodynamic parameter as important as temperature and lately many efforts are paid to use pressure as a new technique of processing of biopolymers as for the food processing. On a molecular basis pressure has been used to study the refolding and dissociation of proteins. These pressure effects are essentially based on the structural perturbation of mostly proteins caused by increasing pressure. The structural perturbation of the enzymes and proteins by pressure is highly dependent on the protein nature and oligomeric compositions.
On the other hand, the pressure effect on enzyme activity is also based on the perturbation on protein structure and protein-ligand interactions, caused by increasing pressure. Sometimes pressure can even affect substrate or reaction specificity of enzymes and such an effect has been uniquely utilized to control some enzyme-catalyzed synthetic reactions by way of pressure.
In this study we proceeded this principle to 1, study of the mechanism of enzyme reactions such as nucleases and proteoses ; 2, control of the productive enzyme reactions by pressure and 3, chemical and biochemical modification of an enzyme under reversible denaturation caused by high pressure.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 功刀 滋: "“Effectof pressure on plant endonuclease reactions"" Nucleic Acids Research. 21. 133-134 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋: "“Pressure as a Control Factor of Enzymatic Synthetic Reactions"," Ann.New York Academy of Science. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋: "“Carboxypeptidaset Yーcatalyzed Reaction in Systems Containing Organic Solvent"," Biocatalysis. 4. 141-151 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋: "“Effect of Pressure on Cyclodextrinーcatalyzed Hydrolysis of Nucleotide 2':3'ーCyclic Monophosphates"," European J.Biochemistry. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋: "“変性剤しての圧力ープロテア-ゼによるタンパク質の限定分解"" 福井大学工学部研究報告. 38. 179-184 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋 (林力丸編): "加圧食品ー研究と開発 (分担分;選択的変性剤としての圧力)" さんえい出版, 374 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 功刀 滋: "加圧食品と高圧生物科学 (分担分;酵素反応と圧力)" さんえい出版, (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] S. Kunugi, K. Iwasaki. A. Nomura: ""Effect of pressure on plant endonuclease reactions"" Nucleic Acids Res., Symp. Ser.21. 133-134 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kunugi, T. Kondoh, H. Hayashiba, A. Nomura: ""Carboxypeptidaset Y-catalyzed Reaction in Systems Containing Organic Solvent"," Biocatalysis. 4. 141-151 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kunugi, A. Nomura: ""Pressure as a Control Factor of Enzymatic Synthetic Reactions"," Ann. New York Acad. Sci.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kunugi, K. Kawade, H. Kabata, A. Nomura, M. Komiyama: ""Effect of pressure on Cyclodextrin-catalyzed Hydrolysis of Nucleotide 2' : 3' -Cyclic Monophosphates"," J. Chem. Soc. Perkin II.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] S. Kunugi, K. Tanabe, T. Kondoh, M. Yokoyama, A. Nomura, N. Itoh, M. Fukuda: ""Study on the inactivation of wheat carboxypeptidase. Implication of the transient active monomer and the half-site reactivity"." Eur. J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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