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1990 Fiscal Year Final Research Report Summary

Research for a Novel Preparation of Liposome Complex Systems (Proteoliposomes) and Their Functions

Research Project

Project/Area Number 01470104
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子物性
Research InstitutionNagoya Institute of Technology

Principal Investigator

TAKIZAWA Akira  Nagoya Institute of Technology, Faculty of Engineering, Department of Materials Science & Engineering, Professor, 工学部, 教授 (90016262)

Co-Investigator(Kenkyū-buntansha) KINOSHITA Takatoshi  Nagoya Institute of Technology, Faculty of Engineering, Department of Materials, 工学部, 講師 (60135407)
TSUJITA Yoshiharu  Nagoya Institute of Technology, Faculty of Engineering, Department of Materials, 工学部, 教授 (70016591)
Project Period (FY) 1989 – 1990
KeywordsPoly (alky1 L-glutamate) / Monolayer Method / hydrolysis / Sequential Polypeptide / Amphiphilic / Micelle / Ion Channel / Artificial Model of Cell Membrane
Research Abstract

Amphiphilic sequential polypeptides whose alpha-helix is hydrophilic on one face and hydrophobic on the opposite face were prepared by a novel and simple technique by using a polypeptide monolayer saponification method. This method involves the formation of a solid condensed monolayer of poly (ganma-methy1 L-glutamate)(PMG), poly (n-hexy1 L-glutamate)(PHeLG) and poly (N^<epsilon>-trifluoroacethy1 L-lysine)(PLys (Tfa)), respectively, at an air-water interface, and a saponification of the polypeptides side chains hydrated in the aqueous phase, keeping the remaining side chains oriented away from the aqueous phase unreacted. The resulting polypeptides, PMG with 34mol% L-glutamic residues, PHeLG with 27mol% L-glutamic acid and PLys (Tfa) with 34mol% L-lysine residues, had the unique amino acid sequence to form an amphiphilic alpha-helix structure. Moreover, the content of L-glutamic acid residue of PMG could be regulated by intruducing dimethylformamide into the aqueous phase at the monolayer saponification reaction. The polypeptides obtained formed a reversed micelle in aqueous solution, on the other hand, in the lipid bilayer membrane the polypeptides formed the cylindrical aggregate consisting of 6 trans-membrane helix rods act as an ion channel through the bilayer membrane.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] M.Higuchi: "“Membrane Potential Induced by Multi Valent Metal ion in a PolypeptideーPhospholipid Composite Membrane"," Rept.Prog.Polym.Phys.,Japan. 32. 671-674 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: "“A.Simple Method for the Preparation of an Amphiphilic sequential Polypeptide"," Macromolecules. 23. 361-365 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: "“Channel Forming Activity of an Amphiphilic Sequential Polypeptide in a Cationic Bilayer Membrane"," Bull.Chem.Soc.Jpn.63. 1916-1920 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: "“Interaction between LーGlutamic Acid Containing Polypeptide and Cu^<2+> ion on a Bilayer Membrane"," Senーi Gakkaishi. 49. 384-390 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Y.Nagata: "“Copper Ion Complex Formation with a Dicarboxylic acidーcontaining Polypeptide"," Rept.Prog.Polym.Phys.,Japan. 33. 555-558 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Higuchi: "“Interaction between an Anionic Amphiphilic Sequential Polypeptide and Anionic Bilaayer Membrane"," Polymer J.23. 15-22 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M. Higuchi: "Membrane Potential Induced by Multi Valent Metal ion in a Polypeptide-Phospholipid Composite Membrane," Rept. Prog. Polym. Phys., Japan,. 32,. 671-674 ((198)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Higuchi,: "A. Simple Method for the Preparation of an Amphilic sequential Polypeptide," Macromolecules,. 23,. 361-365 ((199)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Higuchi,: "Channel Forming Activity of an Amphiphilic Sequential Polypeptide in a Cationic Bilayer Membrane," Bull. Chem. Soc. Jpn.,. 63,. 1916-1920 ((199)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Higuchi,: "Interaction between L-Glutamic Acid Containing Polypeptide and Cu^<2+> ion on a Bilayer Membrane," Sen-i Gakkaishi,. 49,. 384-390 ((199)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Y. Nagata,: "Copper Ion Complex Formation with a Dicarboxylic acid - containing Polypeptide," Rept. Prog. Polym. Phys., Japan,. 33,. 555-558 ((199)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Higuchi,: "Interaction between an Anionic Amphiphilic Sequential Polypeptide and Anionic Bilayer Membrane," Polymer J.,. 23,. 15-22 ((199)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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