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1990 Fiscal Year Final Research Report Summary

Dynamics of Gestalt Polymer Chains in Gel and Separation Mechanism of DNA under Gel Electrophoresis

Research Project

Project/Area Number 01470107
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 高分子物性
Research InstitutionOsaka University

Principal Investigator

KOTAKA Tadao  Osaka Univ. Fac. Science Professor, 理学部, 教授 (20027022)

Co-Investigator(Kenkyū-buntansha) SHIKATA Toshiyuki  Osaka Univ. Fac. Science Research Associate, 理学部, 助手 (10178858)
WATANABE Hiroshi  Osaka Univ. Fac. Science Research Associate, 理学部, 助手 (90167164)
ADACHI Keiichiro  Osaka Univ. Fac. Science Associate Professor, 理学部, 助教授 (00028226)
Project Period (FY) 1989 – 1990
KeywordsDNA / Gel Electrophoresis / Mobility / Polyisoprene / Polybutadiene / Dielectric Relaxation / Polymer Gel / Pin-Down Frequency
Research Abstract

The object of this study was to clarify dynamics of gestalt polymeric chains in gels, and to clarify the mechanism of molecular weight dependent separation in gel electrophersis of DNA. We also attempted to establish the optimum electropheric condition that enabled us to separate enormously large DNA more than 10^6 basepairs (bp). First, we examined dielectric relaxation of gest polyisoprene, PI, that exhibits the dielectric normal mode prucess, dissolved in much longer PI matrix, in polybutadiene (PB) matrix, and also in crosslinked PB matrix with varying molecular weight M. Then, we found that when gest chains were too short to entangle with the gel matrix, their relaxation time was proportinal to M^2, and this could be explained by Rouse-Zimm theory. On the other hand, when the gest chains was so long as to entangle with the gel, the relaxation time was proporinal to M^3, and this well suited with Doi-Edowards theory that is invalid in monodisperse systems.
Then, we proposed a new electric field that has a sinusoidal field with amplitude E_s and frequency f superposed on a steady biase field E_b ; Biased Sinusolidal Field. We found that in a condition of E_b < E_s, DNAs longer than 20 x 10^3 bp had minimum in mobilityat a particular M-dependent frequency, which was called pin-down frequency, f_p. We also confirmed the relation of f_p alpha M^<-1>C_<gel>^<-1>E_S^0E_b^1 from experiments with various agarose gel concentration C_<gel>. Biased reptation model explained molecular weight dependence of mobility in steady field electrophoresis qualitatively, but not quantitatively. More realistic model describing dynamics of large DNA in gel electrophoresis is required for more detailed discussion.

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] Hirotugu YOSHIDA,: "“Dielectric Normal Mode Process of StarーShaped Polyisoprenes"" Polymer Journal. 21. 863-872 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichiro ADACHI: "“Swelling Equilibrium of Solution Crossーlinked Polybutadiene Networks in Polyisoprene Solutions"" Macromolecules. 22. 3106-3111 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichiro ADACHI: "“A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks"" Macromolecules. 22. 3111-3116 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiroshi WATANABE: Polymer Journal. 22. 153-161 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.1.Excluded Volume Effect in Undiluted Binary Blends"" Macromolecules. 23. 2550-2554 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichiro ADACHI: "“Dielectric Normal Mode Process in Binary Blends of Polyisoprene.2.Dynamic Behavior"" Macromolecules. 23. 2554-2559 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirotsugu YOSHIDA: "“Entanglement in Blends of Monodisperes Star and Linear Polystyrenes.2.Concentrated Blends"" Macromolecules. 24. 572-577 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Keiichiro ADACHI: "“Dielectric normalーmode process of polyisoprene in blends and in networks"" Journal of NonーCrystalline solids. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for SizeーDependent DNA Separation"" Biopolymers. 31. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshiyuki SHIKATA: "“Biased Sinusoidal Field Gel Electrophoresis for Large DNA Separation"" Macromolecules. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tadao KOTAKA: "“Structure of multiphase polymers" “Frontiers of Macromolecular Science," IUPAC 32nd Int'l Symposium on Macromolecules August 1ー5,1988,Kyoto." Blackwell Scientific Publications,Oxford & London, (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hirotugu YOSHIDA, Keiichiro ADACHI, Hiroshi WATANABE, and Tadao KOTAKA: ""Dielectric Normal Mode Process of Star-Shaped Polyisoprenes"" Polymer Journal. 21. 863 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichiro ADACHI, Tetsuo NAKAMOTO and Tadao KOTAKA: ""Swelling Equilibrium of Solution Cross-linked Polybutadiene Networks in Polyisoprene Solutions"" Macromolecules. 22. 3106 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichiro ADACHI, Tetsuo NAKAMOTO, and Tadao KOTAKA: ""A Dielectric Study of the Dynamics of Polyisoprene Trapped in Polybutadiene Networks"" Macromolecules. 22. 3111 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiroshi WATANABE, Hirotsugu YOSHIDA, and Tadao KOTAKA: ""Model Calculation for Viscoelastic and Dielectric Relaxation functions of Non-entangled Star Chains with Arm Length Distribution"" Polymer Journal. 22. 153 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichiro ADACHI, Isao NISHI, Shigeru ITOH, and Tadao KOTAKA: ""Dielectric Normal Mode Process in Binary Blends of Polyisoprene. 1. Excluded Volume Effect in Undiluted Binary Blends"" Macromolecules. 23. 2550 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichiro ADACHI, Shigeru ITOH, Isao NISHI, and Tadao KOTAKA: ""Dielectric Normal Mode Process in Binary Blends of Polyisoprene. 2. Dynamic Behavior"" Macromolecules. 23. 2555 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hirotsugu YOSHIDA, Hiroshi WATANABE, and Tadao KOTAKA: ""Entanglement in Blends of Monodisperse Star and Linear Polyrstyenes. 2. Concentrated Blends"" Macromolecules. 24. 572 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Keiichiro ADACHI and Tadao KOTAKA: ""Dielectric normal-mode process of polyisoprene in blends and in networks"" Journal of Non-Crystalline solids. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIKATA and Tadao KOTAKA: ""Biased Sinusoidal Field Gel Electrophoresis for Size-Dependent DNA Separation"" Biopolymers. 31. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toshiyuki SHIKATA and Tadao KOTAKA: ""Biased Sinusoidal Field Gel Electrophoresis for Large DNA Separation"" Macromolecules. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tadao KOTAKA: Blackwell Scientific Publications, Oxford & London. "Structure of multiphase polymers" in T. Saegusa, T. Higashimura, and A. Abe, eda. "Frontiers of Macromolecular Science, " 387 ; IUPAC 32nd Int'l Symposium on Macromolecules August 1-5, 1988. Kyoto., (1989)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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