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1990 Fiscal Year Final Research Report Summary

Ultraviolet Resonance Raman Spectroscopic Study on the Conformation of Enkephalin and Binding Mode to Receptors

Research Project

Project/Area Number 01470142
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Physical pharmacy
Research InstitutionTohoku University

Principal Investigator

TAKEUCHI Hideo  Pharmac. Inst. Asoc. Prof., 薬学部, 助教授 (30111454)

Co-Investigator(Kenkyū-buntansha) HARADA Issei  Pharmac. Inst. Prof., 薬学部, 教授 (70011583)
Project Period (FY) 1989 – 1990
KeywordsEnkephalin / Opioid / Receptor / UV resonance Raman spectroscopy
Research Abstract

We have studied the ultraviolet (213- and 240-nm) resonance Raman (UVRR) spectra of Met-enkephalin, Leu-enkephalin, and [Trp^4] Met-enkephalin incorporated into dilauroylphatidylcholine liposomes. The analysis of the spectra based on the data of model compounds obtained previously has provided several pieces of important information on the binding mode of enkephalin to the lipid bilayers. A delta-type opioid receptor has been extracted from rat brain and purified. The structure of the receptor has been investigated by UVRR and circular dichroism spectroscopy. The results obtained are as follows :
1. Every enkephalin mentioned above exists as an ensemble of various extended conformers in aqueous solution, whereas it takes a folded conformation in the lipid-bound state.
2. The Tyr side chain is inserted into the membrane hydrophobic interior, but the Phe side chain is located in the polar surface region or exposed to the aqueous phase.
3. The degree of insertion of Tyr into the membrane is smaller for Leu-enkephalin than the other two.
4. The deeper the Tyr is buried, the higher the affinity and activity of the peptide.
5. The membrane environment is considered to play a role in protecting the N-terminal Tyr form degradation in tissues.
6. The mainchain of the delta-opioid receptor is largely in the beta-sheet structure and the protein does not contain many aromatic residues, particularly Tyr and Typ.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] H.Takeuchi: "Effects of Hydrogen Bonding on the Tyrosine Raman Bands in the 1300ー1150 cm^<ー1> Region" J.Raman Spectrosc.20. 233-237 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] T.Miura: "Tryptophan Raman Sensitive to Hydrogen Bonding and Side Chain Conformation" J.Raman Spectrosc.20. 667-LMU (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Takeuchi: "Ultraviolet Resonance Raman Spectroscopy of XーProline Bonds: a New Marker Band of Hydrogen Bonding at the Imide C=O Site" J.Raman Spectrosc.21. 509-515 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Takeuchi: "Binding Modes of Enkephalins to Phospholipid Liposomes Studied by Ultスaviolet Resonance Raman Spectroscopy" Eur.J.Biochem.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H.Takeuchi: "Circular Dichroism and Ultraviolet Resonance Raman Spectra of a δーOpioid Receptor from Rat Brain" Biochim.Biophys.Res.Commun.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] H. Takeuchi, N. Wtanabe, Y. Satoh, and I. Harada: "Effects of Hydrogen Bonding on the Tyrosine Raman Bands in the 1300-1150 cm^<-1> region" J. Raman Spectrosc.20. 233-237 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] T. Miura, H. Takeuchi, and I. Harada: "Tryptophan Raman Bands Sensitive to Hydrogen Bonding and Side Chain Conformation" J. Raman Spectrosc.20. 667-671 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeuchi and I. Harada: "Ultraviolet Resonance Raman Spectroscopy of X-Proline Bonds : A New Marker Band of Hydrogen Bonding at the Imide C=O Site" J. Raman Spectrosc.21. 509-515 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeuchi, K. Ohtsuka, and I. Harada: "Binding Modes of Enkephalins to Phospholipid Liposomes Studied by Ultraviolet Resonance Raman Spectroscopy" Eur. J. Biochem.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] H. Takeuchi, K. Ohtsuka, and I. Harada: "Circular Dichroism and Ultraviolet Resonance Raman Spectra of a delta-Opioid Recptor from Rat Brain" Bichim. Biophys. Res. Commun.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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