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1990 Fiscal Year Final Research Report Summary

Effects of Biologically Active Substances on Function and Morphology of Farm Animal Spermatozoa.

Research Project

Project/Area Number 01480088
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 畜産学(含草地学)
Research InstitutionTohoku University

Principal Investigator

MASAKI Junji  Tohoku Univ. Fac. Agric., Professor, 農学部, 教授 (70101152)

Co-Investigator(Kenkyū-buntansha) SASADA Hiroshi  Tohoku Univ. Fac. Agric., Res. Associate, 農学部, 助手 (90158931)
UMEZU Motoaki  Tohoku Univ. Fac. Agric., Res. Associate, 農学部, 助手 (30005649)
SUGAWARA Shichiro  Tohoku Univ. Fac. Agric., Associate Professor, 農学部, 助教授 (80005602)
Project Period (FY) 1989 – 1990
KeywordsSpermatozoa / Seminal plasma / Zona-free hamster egg / Phospholipase A_2 / Maitotoxin / cAMP-dependent protein kinase / cAMP-independent protein kinase / Acrosome reaction
Research Abstract

Effects of three biologically active substances on the function and morphology of mammalian spermatozoa were studied.
Phospholipase A_2, of which activity has been detected in the seminal plasma of bull, goat and human, accelerated acrosome reaction of hamster spermatozoa. Addition of this enzyme to bull epididymal spermatozoa increased the rate of their entry into zona-free hamster egg. This effect was indefinite for bull ejaculated spermatozoa, suggesting that they had already received influence of enzyme from seminal plasma.
Maitotoxin, a toxic and Ca^<2+>-dependent substance of marine origin, was used to examine whether it can induce the acrosome reaction as does Ca ionophore (A23187) in the mammalian spermatozoa. Maitotoxin inhibited motility and removed acrosome in the bull and goat spermatozoa when some 10 ng/ml were added. The less concentration (1-2ng/ml) induced almost normal acrodome reaction of spermatozoa and increased their entry into zona-free hamster egg. These results show that maitotoxin may be useful for inducer of acrosome reaction.
Goat spermatozoa were used to extract cAMP-independent protein kinase and their substrate protein. Analyses of the purified material have shown that the protein kinase is similar to casein kinase II and there are peptides which are phosphorylated by this enzyme.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] 康 〓秀: "ヤギ精子の運動性、呼吸および先体の形態に及ぼすマイトトキシンの影響" 家畜繁殖誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 浜野 光市: "牛精子の先体反応に及ぼすマイトトキシンの影響" 家畜繁殖誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邊 伸也: "ウシ精漿中のホスホリパ-ゼAに関する研究" 東北大学農学研究科博士論文. 1-100 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡邊 伸也: "透明帯除去ハムスタ-卵子内への精子侵入能に及ぼすホスホリパ-ゼA_2の効果" 家畜繁殖誌.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島崎 琢也: "Characterization of casein kinase IIーlike protein kinase and its potential substrates in sperm of sea urchin" Journal of Biochemistry.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 島崎 琢也: "動物精子cAMP非依存性プロテインキナ-ゼの酵素化学的特徴とその生理的役割に関する研究" 東北大学農学研究科修士論文. 1-81 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] KANG Min-Soo,: "Effect of maitotoxin on motility, respiration and acrosome morphology of goat spermatozoa." J. Anim. Reprod.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] HAMANO koichi: "Effect of maitotoxin on acrosome reaction of bull spermatozo." J. Anim. Reprod.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] WATANABE Shinya: "Phospholipase A in bull seminal plasma." Tohoku Univ. Thesis (Doctor Degree, 1-100, 1990).

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] WATANABE Shinnay: "Effect of phospholipase A_2 on sperm entry into zona-free hamster egg." J. Anim. Reprod.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SHIMAZAKI Takuya: "Characterization of casein kinaseII-like protein kinase and its potential substrates in sperm of sea urchin." J. Biochemistry.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] SHIMAZAKI Takuya: "Characterization of cAMP-independent protein kinase of sea urchin and goat spermatozoa and its physiological role." Tohoku Univ. Thesis (Master Degree, 1-81, 1991).

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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