1991 Fiscal Year Final Research Report Summary
Population structure and segregation analysis of multiple markers in domestic animals and poultry.
| Project/Area Number |
01480093
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
畜産学(含草地学)
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| Research Institution | Gifu University |
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Principal Investigator |
NAKAMURA Takao Gifu University Agriculture Professor, 農学部, 教授 (60021698)
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| Co-Investigator(Kenkyū-buntansha) |
DOI Osamu Gifu University Agriculture Assistant Professor, 農学部, 講師 (60180212)
KIMURA Masao Gifu University Agriculture Professor, 農学部, 教授 (10021693)
TANABE Yuichi Azabu University Veterinary Professor, 獣医学部, 教授 (30021679)
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| Project Period (FY) |
1989 – 1991
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| Keywords | Chicken DNA fingerprinting / Dog MtDNA / RFLP / Polymorphism in MtDNA / Population structure in dog |
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| Research Abstract |
Developments in molecular genetics focused interest on a new class of genetic markers. The technique of DNA fingerprinting(DFP)has provided a method of comparing DNA characteristics of individuals.
For obtaining clear DNA fingerprint and detecting variation with the bacteriophage M 13 probe in chickens, parentage test was carried out with fingerprints of White Leghorn, Nagoya, Mikawa species and their F1 offspring. All bands of F1 were always originated from either parent. DNA fingerpriuting always of their breeds(White Leghorn, Nagaya, Mikawa)and synthetic chicken showed that their banding pattern is specific to each individual. Comparison with these banding patterns suggested the pattern is more similar within breeds than between breeds. Thus, DNA fingerprinting is useful not only for identifying the individual and parent-offspring but also for classifying the breeds of chickens.
To elucidate phylogenetic relationships of various dog breeds and populations mtDNA restriction enzyme cleavage(RFLP)patterns were employed. After DNAs were extracted from blood, southern blood hybridization was performed. The intrabreed variation of the pattern was found in Ryukyu dogs, Chejudo dogs, Siba dogs, Beagles and German Shephereds by Apa I and Eco RV used as restriction enzymes. By use of four-base restriction enzyme Hint 1, the inter-breed and intra-breed variations were found in Beagles and German Shepherds, and the inter-breed variation was found in Chow Chow and pointer. The comparison of mtDNA cleavage patterns among Japanese dogs, Asian(except Japan)dogs and European dogs show no consistent trend among the patterns.
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