• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1991 Fiscal Year Final Research Report Summary

Histochemical methods using physical development procedures for glycoconjugates and their applications

Research Project

Project/Area Number 01480109
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General anatomy (including Histology/Embryology)
Research InstitutionNagoya City University

Principal Investigator

YAMADA Kazuyori  Nagoya City University Medical School, 2nd Department of Anatomy, Full Professor, 医学部, 教授 (10022766)

Co-Investigator(Kenkyū-buntansha) HIRABAYASHI Yoshifumi  Nagoya City University Medical School, 2nd Department of Anatomy, Research Assoc, 医学部, 助手 (30181184)
FUJIMORI Osamu  Nagoya City University Medical School, 2nd Department of Anatomy, Assistant Prof, 医学部, 講師 (30128350)
KOSHIBA Kimikazu  Nagoya City University Medical School, 2nd Department of Anatomy, Associate Prof, 医学部, 助教授 (30080055)
Project Period (FY) 1989 – 1991
KeywordsGlycoconjugates / Physical development / Histochemistry / Methods of detection / Chemical groupings / Chemical modifications / Enzyme digestions / Applications to various organs of animals
Research Abstract

Attempts have successfully been made to introduce physical development (silver enhancement procedures into the theoretical and applied histochemistry of glycoconjugates at light and electron microscopical levels.
(A) In the theoretical histochemistry of glycoconjugates, at least three aspects of the research results were obtained.
(1) In light and electron microscopy, histochemical methods using physical development procedures were derived and established for the detection of vicinal diols of glycoconjugates, such as periodic acid (PA)-thiocarbohydrazide (TCH)-silver protein (SP)-physical development (PD) and PA-TCH-potassium trichloro (ethylene) platinum (KTP)-sodium borohydride (BH)-PD.
(2) In light and electron microscopy, histochemical methods using physical development procedures were derived and established for the detection of acidic (sulfate and/or carboxyl) groupings of glycoconjugates, such as high or low iron diamine (HID or LID)-TCH-SP-PD, HID or LID-KTP-BH-PD and alcian blue … More (AB) pH 1.0 or 2.5-KTP-BH-PD.
(3) In light and electron microscopy, histochemical methods using physical development procedures were derived and established for the detection of saccharide residues of glycoconjugates, such as lection (concanavalin A, Ricinus communis agglutinin-I, Wheat germ agglutinin etc.) (LT)-gold (G)-PD, LT-immunogold (IG)-PD and LT-anti-lectin (Anti-LT)-protein A gold (PAG)-PD.
Throughout (1)-(3), the specificity, reliability and reproductibility of each method were substantiated by means of appropriate control procedures, stainings of model substances and chemical modification and enzyme digestion procedures.
(B) In the applied histochemistry of glycoconjugates, all the histochemical methods established in (A) have been applied to a series of mammalian organ systems, such as nervous, circulatory, endocrine, reproductive, digestive and respiratory systems. In various cells and tissues involved in the organs of these systems, the localizations and histochemical properties of glycoconjugates were precisely determined and these contained either hitherto unknown new findings or much better findings than those obtained hitherto.
Taken altogether, it can be stated conclusively that all the results obtained in the present project could open new avenues in the theoretical and applied histochemistry of glycoconjugates in general. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 保木本 誠,藤森 修,山田 和順: "ラット舌の組織化学的研究" 日本解剖学会第51回中部地方会プログラム・予稿集. 60-61 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Hiromi Ueda,Yoshishige Yanase,Osamu Fujimori & Kazuyori Yamada.: "Cytochemical studies on vicinal diol-containing carbohydrates in the endothelium of the rat splenic blood vessels." Nagoya Medical Journal. 36. 115-127 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 平林 義章,磯貝 文典,山田 和順: "光顕組織化学における酵素消化法の検討" 結合組織. 23(Suppl). 60 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 保木本 誠,藤森 修: "ラットおよびモルモットの舌の組織化学的研究" 名古屋市立大学医学会雜誌. 43. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyori Yamada: "Histochemistry of carbohydrates as performed by means of physical development procedures" Histochemical Journal. 24. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Osamu Fujimori & Kazuyori Yamada: "Lectin-immunogold-silver staining methods for glycoconjugate histochemistry" Journal of Histochemistry and Cytochemistry. 40. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 山田 和順: "光顕レベルの組織化学的諸方法の基本 組織細胞化学1991(日本組織細胞化学会編)" 学際企画, 1-21 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 横田 貞記,藤森 修: "イムノゴ-ルド法ーコロイド金による免疫組織化学ー" ソフトサイエンス社, 1-258 (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kazuyori Yamada and Yoshifumi Hirabayashi: "VIII Histochemistry of glycoproteins. 23 Light microscopy." New Lecture Series of Biochem. Exper., 3 Carbohydrates I Glycoproteins (ed. Jap. Soc. Biochem.). Tokyo kagaku Dohjin. 763-772 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Hirabayashi and Kazuyori Yamada: "Methods for the histochemical detection of acidic glycoconjugates by means of sensitized alcian blue procedures." Acta Anat. Nippon.65(4). 254 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshikazu Abe, Hiromi Ueda and Kazuyori Yamada: "Electron microscopic cytochemistry of sulfated and neutral carbohydrates in the endothelial cells lining the capillaries of the rat salivary glands." J. Histochem. Cytochem.38. 1049 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takashi Ueda, Kimikazu Koshiba and Kazuyori Yamada: "Histochemical studies on neutral carbohydrates in the rat circumventricular organs." Acta Anta. Nippon.65(6). 487 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiromi Ueda, Yoshishige Yanase, Osamu Fujimori and Kazuyori Yamada: "Cytochemical studies on vicinal diol-containing carbohydrates in the endothelium of the rat splenic blood vessels." Nagoya Med. J.36. 115-127 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hiromi Ueda, Hirokazu Kitamura and Kazuyori Yamada: "Electron microscopic histochemical studies on acidic glycoconjugates in the blood vascular system of the rat spleen." Acta Histochem. Cytochem.24. 536 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Hirabayashi and Kazuyori Yamada: "Efficient histochemical methods for the detection of acidic glycoconjugates by means of sensitized diamine procedures." Connective Tissue. 22(1). 39-40 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Noriharu Shibata, Yoshifumi Hirabayashi and Kazuyori Yamada: "Light microscopic histochemistry by means of diamine-thiocarbohydrazide-silver protein-physical development procedures." Acta. Anat. Nippon.66(4). 302 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu Fujimori: "Immunogold-silver staining methods." Immunogold Mehods - Immuno-Histochemistry by means of Colloidal Gold - (eds. S. Yokota & O. Fujimori). Softscience Co.83-102 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu Fujimori: "Double staining methods." Immunogold Methods - Immuno-Histochemistry by means of Colloidal Gold - (eds. S. Yokota & O. Fujimori). Softscience Co.103-110 (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshifumi Hirabayashi: "Light microscope detection of acidic glycoconjugates with sensitized diamine procedures." Histochemical J.24. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kazuyori yamada: "Histochemistry of carbohydrates as performed by means of physical development procedures." Histochemical J.24. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Makoto Hokimoto and Osamu Fujimori: "Histochemical studies on the tongue of the rat and guinea pig." J. Nagoya City Univ. Med. Assoc.43. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Osamu Fujimori and Kazuyori Yamada: "Lectin-immuno-gold-silver staining methods for glycoconjugate histochemistry." J. Histochem. Cytochem.40. (1992)

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-03-16  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi