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1990 Fiscal Year Final Research Report Summary

Stimulus-Secretion Coupling : Studies on Intracellular Ca^<2+> Effect and Its Modulating Factors

Research Project

Project/Area Number 01480140
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General pharmacology
Research InstitutionThe University of Tokushima

Principal Investigator

OKA Motoo  The University of Tokushima Professor School of Medicine, 医学部, 教授 (60028298)

Co-Investigator(Kenkyū-buntansha) ISHIMURA Yasuko  The University of Tokushima Professor School of Medicine Assistant, 医学部, 助手 (40035431)
MORITA Kyoji  The University of Tokushima Professor School of Medicine Associate Professor, 医学部, 助教授 (50108883)
Project Period (FY) 1989 – 1990
KeywordsStimulus-secretion coupling / Ca^<2+> / Regulation of Ca^<2+> effect / Adrenal chromaffin cell / Cytoskeletal element / Protein Kinase / Ba^<2+>
Research Abstract

We have investigated the effects of drugs on catecholamine (CA) secretion from adrenal chromaffin cells, used as a model of catecholaminergic neurons, to clarify the role of Ca^<2+> in secretory mechanism and modulating factors of the intracellular Ca^<2+> effect.
1) ML-9, a perferential inhibitor of myosin ligho-chain kinase (MLCK), inhibited CA secretion stimulated by ACh, high K^+, veratridine or palytoxin by inhibiting Ca^<2+> uptake into the cells, suggesting that MLCK is involved in the secretory mechanism by regulating transmembrane Ca^<2+> uptake. Palytoxin, a potent marine toxin, was found to stimulate CA secretion by activation of Na^+-dependent, TTX insensitive voltage dependent Ca^<2+> channels. Histamine and bradykinin stimulated CA secretion by increasing Ca^<2+> uptake into the cells. Most of the VIP was localized with ACh in the splanchnic nerve terminals and release with ACh and regulated the stimulatory effect of ACh on CA secretion.
2) From the investigation using cytochalasin, vinblastine or actin-like protein, we have proposed the possibility that cytoskeletal elements play an important role in the regulation of tyrosine uptake by the cells, tyrosine hydroxylase (CA synthesis) and CA secretion. The inhibitory effect of flavonoids, Protein kinase C inhibitors, and the stimulatory effect of phorbol ester and Ba^<2+> on CA biosynthesis and secretion suggested the possible involvement of PKC as modulator of secretory mechanism as well as CA biosynthesis.

  • Research Products

    (10 results)

All Other

All Publications (10 results)

  • [Publications] Manabu,Yoshikawa: "Localization and release of immunoreactive VIP in bovine adrenal medulla" Neurosci.Lett.111. 75-79 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyoji,Morita: "Effects of bioflavonoids on exocytotic release of catecholamine from digitoninーpermeabilized chromaffin cells" Neurochem.Int.16. 313-318 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kyoji,Morita: "Effects of bioflavonoids on catecholamine biosynthetic activity in the adrenal gland" Neurochem.Int.17. 21-26 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shuichi,Hamano: "Pharmacological study on Ba^<2+>ーStimulated catecholamine secretion from cultured bovine adrenal chromaffin cell" Jpn.J.Pharmacol.55. 43-50 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Atsushi,Nakanishi: "Palytoxin:A potent stimulator of catecholamine release from cultured bovine adrenal chromaffin cell" Neurosci.Lett.121. 163-165 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshikawa, M., Saito, H., Sano, T., Ohuchi, T., Ishimura, Y., Morita, K., Saito, S. and Oka, M.: "Localization and release of immunoreactive vasoactive intestinal ploypeptide in bovine adrenal madulla." Neurosci. Lett.111. 75-79 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, K., Hamano, S., Teraoka, K., Oka, M. and Yoshizumi, M.: "Effects of bioflavonoids on exocytotic release of catecholamine from digitonin-permeabilized chromaffin cells : A comparison with the effects of other protein kinase C inhibitors." Neurochem. Int.16. 313-318 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morita, K., Teraoka, K., Hamano, S., Oka, M. and Azuma, M.: "Effects of bioflavonoids on catecholamine biosynthetic activity in the adrenal gland : in vitro studies using partially purified tyrosine hydroxylase and chromaffin cell cultures." Neurochem. Int.17. 21-26 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hamno, S., Morita, K., Azuma, M., Oka, M_<2+>and Teraoka, K.: "Pharmacological study on Ba^<2+>- stimulated catecholamine secretion from cultured bovine adrenal chromaffin cells : Possible relation of Ba^<2+> action to Ca^<2+>-activated secretory mechanism." Jap. J. Pharmacol.55. 43-50 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nakanishi, A., Yoshizumi, M., Morita, K., Murakumo, Y., Houchi, H. and Oka, M.: "Palytoxin : A potent stimulator of catecholamine release from cultured bovine adrenal chromaffin cells." Neurosci. Lett.121. 163-165 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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