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1990 Fiscal Year Final Research Report Summary

The Role of Prostaglandins in Learning and Memory

Research Project

Project/Area Number 01480151
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field General medical chemistry
Research InstitutionOsaka Bioscience Institute

Principal Investigator

WATANABE Yasuyoshi  Osaka Bioscience Institute, Department of Neuroscience, Head, 第3研究部, 研究部長 (40144399)

Co-Investigator(Kenkyū-buntansha) MORI Kensaku  Osaka Bioscience Institute, Department of Neuroscience, Vice-Head, 第3研究部, 副部長 (60008563)
Project Period (FY) 1989 – 1990
KeywordsProstaglandin / Memory / Learning / Hippocampus / Long-term Potentiation / Prostaglandin Endoperoxide Synthase / Phospholipase A2 / Microdialysis
Research Abstract

1. Localization of prostaglandin endoperoxide synthase in the hippocampus
Immunohistochemical localization of Prostaglandin Endoperoxide Synthase (PES) in monkey hippocampus was investigated by the use of monoclonal antibody against purified PES. PES-immunoreactivity was intense in the neurons in the hippocampu and cerebral cortex. Especially, the granular and pyramidal neurons in the dentate gyrus and Ammon's horn of the hippocampus showed the most intense immunoreactivity. In the pyramidal cells in CA3 region, positive staining was seen the apical dendrites as well as the proximal dendrites and somata.
2. Effects of PGs on long-term potentiation in the CA3 neurons of guinea pig hippocamp slice
The experimental set-up for long-term potentiation was established by using hippocampal slices of guinea pig. Prostaglandin (PG) s D2, E2, and F2alpha upto 20 muM ha no effects on excitatory post-synaptic potential and long-term potentiation of CA3 neurons by tetanic stimuli of dentate gyrus.
3. Novel cytosolic phospholipase A2 in the rat brain and its translocation
When phospholipase A2 (PLA2) activities were investigated in the rat brain, some activities with the major two peaks on the gel filtration column were found in the cytosolic fraction of the brain. Low molecular-weight form (PLAL) was active at neutral pH and absolutely required Ca^<2+> for its activity. In the reconstitution syste of partially purified PLAL and synaptosomal membranes from rat brain, PLAL associated with the membranes in a Ca^<2+>-dependent manner.
4. Effect of PGE2 on the glutamate release
By using microdialysis technique, we investigated the effect of PGE2, whose receptor enriched in the hippocampus, on the release of glutamate which is considered to be major trigger for long-term potentiation in the hippocampus. PGE2 enhanced glutamate release in several portions of the brain.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] 坪倉 省吾、渡辺 恭良、江原 寛昭、山本 尚三、鏡山 博行、早石 修: "サル脳における Prostaglandin Endoperoxide Synthase の局在" 第13回神経科学学術集会予稿集. 174

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsubokura, S., Watanabe, Y., Ehara, H., Imamura, K., Sugimoto, O., Kagamiyama,H., Yamamoto, S., and Hayaishi, O.: "Localization of prostaglandin endoperoxide synthase in neurons and glias in monkey brain." Brain Res.543. 15-24 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Morii, H., and Watanabe, Y.: "Solubilization of prostaglandin D2 binding protein from porcine temporal cortex." Biochem. Biophys. Res. Commun.174. 364-371 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Islam, F., Watanabe, Y., and Hayaishi, O.: "Inhibition of rat brain prostaglandin D synthase by 3ーhydroxyー3methylglutaryl coenzyme A reductase inhibitors." Biochem.Internatl.22. 601-605 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsumura, K., Watanabe, Yu.,Onoe, H., Watanabe, Y., and Hayaishi, O.: "High density of prostaglandin E2 binding sites in the anterior wall of the 3rd ventricle: a possible site of its hyperthermic action." Brain Res.553(1). 147-151 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshihara, Y., and Watanabe, Y.: "Translocation of phospholipase A2 from cytosol to membranes in rat brain induced by calcium ions." Biochem. Biophys. Res. Commun.170(2). 484-490 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tsubokura, S., Watanabe, Y., Ehara, H., Imamura, K., Sugimoto, O., Kagamiyama, H., Yamamoto, S., and Hayaishi, O.: "Localization of prostaglandin endoperoxide synthase in neurons and glias in monkey brain" Brain Res.543. 15-24 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morii, H., and Watanabe, Y.: "Solubilization of prostaglandin D_2 binding protein from porcine temporal cortex" Bio chem. Bio phys. Res. Commun.174. 364-371. (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Islam, F., Watanabe, Y., and Hayaishi, O.: "Inhibition of rat brain prostaglandin D synthase by 3-hydroxy-3methylalutaryl coenzyme A reductase inhibitors" Bio chem. International.22. 601-605 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsumura, K., Watanabe, Yu., Onoe, H., Watanabe, Y., and Hayaishi, O.: "High density of prostahlandin E2 binding sites in the anterior wall of the 3rd ventricle : a possible site of its hyperthermic action" Brain, Res.553 (1). 147-151. (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshihara, Y., and Watanabe, Y.: "Translocation of phospholipase A2 from cytosol to membranes in rat brain induced by calcium ions" Bio chem. Bio phys. Res. Commun.170 (2). 484-490. (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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