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1991 Fiscal Year Final Research Report Summary

Axonal and nueronal degeneration in relation to balance changes in synthesis, transport and degradation of cytoskeletal proteins.

Research Project

Project/Area Number 01480152
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Pathological medical chemistry
Research InstitutionGunma University

Principal Investigator

KOMIYA Yoshiaki  Department of Molecular and Cellular Neurobiology, Gunma University School of Medicine Professor, 医学部, 教授 (50010046)

Project Period (FY) 1989 – 1991
KeywordsCytiskeletal proteins / Axonal transport / Neuronal aging / Neuronal regeneration / Tubulin / Actin / Neurofilament / Microtubules
Research Abstract

Dynamic organization and transport of axonal cytoskeletal proteins were investigated using rat dorsal root ganglion cell and ventral horn motoneuron systems, and the following results were obtained.
1. Three subpopulations of axonal cytoskeleton were distinguished by their solubility properties in 1% cold Triton X-100 containing buffer ; stably polymerized, dynamic and depolymerized froms. The last form was thought to be transported form.
2. Two subcomponents of slow axonal transport were distinguished by their ratc and composition ; slow component a and b (Sca and Scb respectively). The formerconsisted mainly of stably polymerized form and the latter of dynamic form. The average rate of slow axonal transport slowed down with age.
3. Separation into Sca and Scb was seen in young adult rat, e. g. 7weeks of age or older, a few weeks after labelling, but not in young rat, e. g. 4 weeks of age. In older rat than 30 weeks, however, a large peak of stably polymerized form appeared at the initia … More l portion of axon, which scarecely migrated distally. From the accumulated peak of stably polymerized form, a population of dynamic form came out during a next few weeks, which was not reorganized into stably polymerized cytoskletons as in the case of young rat. This resulted in functional denervation of distal axon, probably causing axonal and neuronal degeneration.
4. From the results above mentioned, it is suggested that the rate of interconversion among three subpopulations of axonal cytosksleton slows down with age, and especially in the older rat, reorganization from dynamic to stably polymerized forms is severely affected. The dynamic form which fails to reconstitute stably polymerized form may be degraded.
5. When axon was injured, it quickly respoded to increase dynamic subpopulation along its whole length for repairing the injury. At the same time the rate of slow axonal transport increased, and the synthesis of tubulin and actin in the neuronal cell body also increased after a while.
6. The axonal cytoskeleton is not a rigid structure as ever thought, but rather a dynamic one responding promptly to its environmental conditions. Less

  • Research Products

    (16 results)

All Other

All Publications (16 results)

  • [Publications] Tashiro,T.& Komiya,Y.: "Stable and dynamic forms of cytoskeletal proteins in slow axonal transport." journal of Neuroscience. 9. 760-768 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Zimmermann,H.,Tashiro,T.,Komiya,Y.& Kurokawa,M.: "Axonal transport studied in a single vertebrate neuron:the giant electromotor neuron of the electric catfish,Malapterurus electricus." Nueroscience Research. 6. 248-256 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro,T.& Komiya,Y.: "Changes in organization of cytoskeletal proteins during regeneration." Journal of Neurochemistry. 56. 1557-1563 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Sekimoto,S.,Tashiro,T.& Komiya,Y.: "Two 68kDa proteins in slow axonal transport belonging to 70kDa heat shock protein family and annexin family." journal of Neurochemistry. 56. 1774-1782 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro,T.& Komiya,Y.: "Maturation and aging of the axonal cytoskeleton:biochemical analysis of transported tubulin." Journal of Neuroscience Research. 30. 192-200 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Igarashi,M.& Tashiro,T.& Komiya,Y.: "Actin-binding proteins in growth cone particles(GCP)from fetal rat brain:a 44kDa actin-binding protein is enriched in the fatal GCP fraction." Developmental Brain Research. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Komiya,Y.& Tashiro,T.(Nagatsu,T.et al.eds.,): "Basic,Clinical and Therapeutic Aspects of Alzheimer's and parkinson's Diseases." Plenum Press, 187-190 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Umeda,T.,Tashiro,T.,Komiya,Y.,Onodera,K.& Ishibashi,Y.(Ishibashi,Y.& Hori,Y.eds.): "Tuberous Sclerosis and Neurofibromatosis:Epidemiology,Pathophysiology,Biology and management." Elsevier Science Publishers B.V., 77-84 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tashiro, T. & Komiya, Y.: "Stable and dynamic forms of cytoskeletal proteins in slow axonal transport." Jouranal of Neuroscience. 9(3). 760-768 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Zimmermann, H., Tashiro, T., Komiya, Y. & Kurokawa, M: "Axonal transport studied in a single vertebrate neuron the giant electromotor neuron of electric catfish : Malapterurus electricus." Neuroscience Research. 6(3). 248-256 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, T. & Komiya, Y.: "Changes in organization and axonal transport of cytoskseltal proteins during regeneration." Joural of Neurochemistry. 56(5). 1557-1563 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sekimoto, S., Tashiro, T. & Komiya, Y: "Two 68-kDa proteins in slow axonal transport belong to the 70-kDa heat shock protein family and the annexin family." Journal of Neurochemistry. 56(5). 1774-1782 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tashiro, T. & Komiya, Y.: "Maturation and aging of the axonal cytoskeleton : biochemical analysis of transported tubulin." Journal of Neuroscience Research. 30(1). 192-200 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Igarashi, M., Tashiro, T. & Komiya, Y.: "Actin-binding Proteins in growth cone particles (GCP) from fetal rat brain : a 44 kDa actin-binding protein is enriched in the fetal GCP fraction." Developmental Brain Research. (1992)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Komiya, Y. & Tashiro, T.: "Age-related changes in the rate and composition of the slow axonal transport." Basic Clinical and Therapeutic Aspects of Alzheimer's and Parkinson's Diseases.Plenum Press. 187-190 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Umeda, T. Tashiro, T., Komiya, Y., Onodera, K. & Ishibashi, Y.: "Cytoskeletal 55 kDa protein specific to cultured cells from angiblastoma of tuberous sclerosis." Tuberous Sclerosis and Neurofibromatosis : Epidemiology, Pathophysiology, Biology and Manegement.Elsevier Science Publishers. 77-84 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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