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1990 Fiscal Year Final Research Report Summary

Suppressive Effects of Nude Gene on Thymomagenesis in BUF/Mna Rats.

Research Project

Project/Area Number 01480168
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Experimental pathology
Research InstitutionNagoya University

Principal Investigator

MATSUYAMA Mutsushi  Nagoya University School of Medicine, Professor, 医学部, 教授 (80073112)

Co-Investigator(Kenkyū-buntansha) KOJIMA Akinori  Aichi Cancer Center Research Institute, Principal Investigator, 主任研究員 (60073136)
Project Period (FY) 1989 – 1990
KeywordsBUF / Mna rat / Thymoma / Rat nude gene / BUF / Mna-rnu rat / Suppression of thymomagenesis
Research Abstract

1. Examination on suppression of thymomagenesis in BUF/Mna-rnu/+ rats : (1) A congenic nude rat strain was established by 20 sequential backcrossings of BUF/Mna rats to (N : NIH-rnu/+ X BUF/Mna) F1 rats. (2) A large decrement of thymus weight by the introduction of rnu gene was confirmed in rats sequentially backcrossed for 19 generations. Furthermore, a complete suppression of thymomagenesis was reconfirmed in 18 months old BUF/Mna-rnu/+rats sequentially backcrossed for 17 generations.
2. (1) We examined the expression of receptor-type tyrosine kinase genes in seven cell lines which were established from benign (2 lines) or malignant (2 lines) thymomas of BUF/Mna rats and normal thymic epithelial cells of ACI/NMs rats. Poly A^+ RNAs were purified from these cell lines and analyzed by Northern blotting using EGFR, c-erbB-2, insulin receptor, ret and kit cDNAs as probes. As a result, only the EGFR gene was found to be expressed in all cell lines, although the level of its expression in malignant thymoma cell lines was several-fold lower than that in benign thymoma and normal thymic epithelial cell lines. Two major transcripts of 10 and 5.8 kb, which were the same as those observed in normal liver RNA, were detected in them. (2) We isolated 17 clones of 92/93bp monomer and 8 clones of 185bp dimer of satellite I DNA from thymoma DNAs of BUF/Mna rats. Based on the nucleotide sequences, 16 clones of the monomer were classified into four subgroups (A, B, C and D) and 8 clones of the dimer into three (AB, BC, and CD). However, one clone of the monomer was unclassifiable. Nucleotide sequence indicated that this clone was generated by DNA deletion which occurred during unequal sister chromatid exchange and by gene conversion between subgroups of the monomer.

  • Research Products

    (23 results)

All Other

All Publications (23 results)

  • [Publications] Howie.A.J.: "Different types of segmental sclerosing glomerular lesions in six experimental models of proteinuria." J.Path.157. 141-151 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ogiu,T.: "Thymic lymphomas induced by NーpropylーNーnitrosoures(PNU)in the BUF/Mna rat.an inbred strain with a high icidence of spontaneous thymoma." Acta Path.Jpn.39. 706-711 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 松山 睦司: "胸腺腫" 現代医学. 37. 213-220 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tominaga,K.: "Effects of cholinergic agonists on the proliferation and protein synthesis in acultured thymic epithlial cell line." Cell.Molec.Biol.35. 679-686 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama.M.: "Genetic regulation of the development of glomerular sclerotic lesions in the BUF/Mna rat." Nephron. 54. 334-337 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Masuda,A.: "Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus." In Vitro Cell Devel.26. 713-721 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita,Y.: "New thymocyte growth factor from thymic epithelial cell line." Cell.Mol.Biol.36. 429-438 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Msauda,A.: "Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus." In Vitro Cell Develop.Biol.26. 713-721 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kinoshita,Y.: "New thymocyte growth factor from thymic epithelial cell line." Cell.Mol.Biol.36. 429-438 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama,M.: "Genetic regulation of the development of glomerular sclerotic lesions in the BUF/Mna rat." Nephron. 54. 334-337 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama,M.: "B.Wu and J.Zheng(eds.),ImmuneーDeficient Animals in Experimental Medicine." Karger,Basel, 5 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Kojima,A.: "B.Wu and J.Zheng(eds.),ImmuneーDeficient Animals in Experimental Medicine." Karger,Basel, 6 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Matsuyama,M.: "T.C.Jones,J.M.Ward,U.Mohr,and R.D.Hunt(eds.),Monographs on Pathogy of Laboratory Animals. Hematopoietic System." SpringerーVerlag,Berlin, 6 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Howie, A. J., et al. :"Different types of segmental sclerosing glomerular lesions in six experimental models of proteinuria." J. Path.,. 157 :. 141-151, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ogiu, T., et al. :"Thymic lymphomas induced by N-propyl-N-nitrosourea (PNU) in the BUF/Mna rat, an inbred strain with a high incidence of spontaneous thymoma." Acta Path. Jpn.,. 39 :. 706-711, (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, M. :"Thymoma. (in Japanese)." Gendai-igaku,. 37 :. 213-220 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tominaga, K., et al. :"Effects of cholinergic agonists on the proliferation and protein synthesis in a cultured thymic epithelial cell line." Cell. Molec. Biol.,. 35 :. 679-686 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, M., et al. :"Genetic regulation of the development of glomerular sclerotic lesions in the BUF/Mna rat." Nephron. 54 :. 334-337, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Masuda, A., et al. :"Establishment of functional epithelial cell lines from a rat thymoma and a rat thymus." In Vitro Cell Devel.26 :. 713-721 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kinoshita, Y., et al. :"New thymocyte growth factor from thymic epithelial cell line." Cell. Mol. Biol.36 :. 429-438, (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, M., et al,: "Establishment of a congenic nude strain of rats, BUF/Mna-rnu : Suppression of thymomagenesis in hetrozygous rats." Basel : Karger.27-31 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kojima, A., et al.: "Single dosage effects of the nude gene." Basel : Karger.32-37 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Matsuyama, M.: "Thymoma, lymphocytic, thymus, rat" Berlin : Springer-Verlag,. 275-280 (1990)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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