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1991 Fiscal Year Final Research Report Summary

Monoclonal Antibodies as a Diagnostic Tool for Helminth Infection

Research Project

Project/Area Number 01480170
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 寄生虫学(含医用動物学)
Research InstitutionYamagata University

Principal Investigator

SENDO Fujiro  Yamagata University, Parasitology, Professor, 医学部, 教授 (80091833)

Co-Investigator(Kenkyū-buntansha) SAITO Susumu  Yamagata University, Parasitology, Associate Professor, 医学部, 助教授 (50034004)
Project Period (FY) 1989 – 1990
Keywordsmonoclonal antibody / Schistosoma japonicum / Gnathostoma spinigerum / Trichinella spiralis / Trypanosoma cruzi / specific monoclonal antibody / Polyspicific monoclonal antibody / heterophile monoclonal antibody
Research Abstract

The fundamental tool for diagnosis of parasite infection is egg detection by fecal examination. However, in some parasite infection, it is impossible to detect eggs in the faces because of the specific life history of the worm. Furthermore, in endemic areas, it is sometimes difficult to find specialists skilled in microscopic diagnosis. For these reasons, immuno-sero diagnosis may be a useful approach, but as is well known, crude parasite antigens always show some degree of cross-reactivity with heterologous parasite antigens. To overcome this problem, purification of these antigens is critical. Affinity chromatography using a monoclonal antibody (MoAb) to a specific parasite appears a promising aid to diagnosis. Furthermore, production of recombinant protein using monoclonal antibody as a probe may be useful not only for diagnosis but for the development of a vaccine against parasite infection. With these points in mind, we attempted to establish MoAbs to various helminths as the first step to purification of diagnostic antigens.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] Wichit Rojekittikhun,Susumu Saito,Takao Yamashita,Tadashi Watanabe,Prasert Setasuban.and Fujiro Sendo: "Three Monoclonal Antibodies against Advanced Thirdstage Larvae of Gnathostoma spinigerum" Japanese Journal of Parasitology. 40. 287-292 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Pen Gao, Susumu Saito,Takao Yamashita,Tadashi Watanabe and Fujiro Sendo: "Characterization of a Monoclonal Antibody that specifically recognizes a target antigen in Schistosoma japonicum a dult worms." American Jouranal of Tropical Medicine & Hygiene.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Susumu Saito,Takao Yamashita,Pen Gao,Wichit Rojekittikhun and Fujiro Sendo: "Autoimmunity of hybridoma produced from spleen cells of BALB/c mice infected or immunized with parasites." Parasitological Immunology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Susumu Saito,Takao Yamashita,Pen Gao,Wichit Rojekittikhun,and Fujiro Sendo: "Production and properties of the immunized with the muscle larval antigen of Trichinella spiralis." Parasitological Immunology.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Wichit Rojekittikun, Susumu Saito, Takao Yamashita, Tadashi Watanabe, and Fujiro Sendo: "Three Monoclonal Antibodies against Advanced Thirdstage Larvae of Gnathostoma spinigerum" Japanese Journal of Parasitology. 40. 287-292 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Pen Gao, Susumu Saito, Takao Yamashita, Tadashi Watanabe, and Fujiro Sendo: "Characterization of a Monoclonal Antibody that specifically recognizes a target antigen in Schistosoma japonicum adult worms" American Journal of Tropical Medicine & Hygiene.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Susumu Saito, Takao Yamashita, Pen Gao, Wichit Rojekittikhun, and Fujiro Sendo: "Autoimmunity of hybridoma produced from spleen cells of BALB/c mice infected or immunized with parasites" Parasitological Immunology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Susumu Saito, Takao Yamashita, Pen Gao, Wichit Rojekittikhun, and Fujiro Sendo: "Production and properties of the immunizde with the muscle larval antigen of Trichinella spiralis" Parasitological Immunology.

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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