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1991 Fiscal Year Final Research Report Summary

Study on virulence factors of Vibrio parahaemolyticus

Research Project

Project/Area Number 01480179
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 細菌学
Research InstitutionOsaka University

Principal Investigator

HONDA Takeshi  Res. Inst. for Microb. Dis., Osaka Univ. ; Professor, 微生物病研究所, 教授 (60029808)

Co-Investigator(Kenkyū-buntansha) YOH Myonsun  Res. Inst. for Microb. Dis., Osaka Univ. ; Instructor, 微生物病研究所, 助手 (70093482)
YAMAMOTO Koichiro  Res. Inst. for Microb. Dis., Osaka Univ. ; Assoc. Prof, 微生物病研究所, 助教授 (30158274)
Project Period (FY) 1989 – 1991
KeywordsVibrio Parahaemolyticu / Vp-TDH / Vp-TRH / Hemolysin / Colonization / Monoclonal Antibody
Research Abstract

In this study, we investigated the pathogenic mechanism of gastroenteritis due to Vibrio parahaemolyticus. Following results were obtained through this experiment.
1) A Vp-TDH-related hemolysin (named VP-TRH) was discovered as a virulence factor from human isolates of Kanagawa phenoffienon-negative V. parahaemolyticus
2) Production of Vp-TDH/TRH-related toxins by V. choleras non-51, V. mimicus, and V. hollisae was demonstrated. These VP-TDH/TRH family toxins were purified
3) and iharacterized. Gene cloning of these toxins was also performed.
3) Immunological properties of VP-TDH/TRH were analysed by both monoclonal and polyclonal antibodies.
4) Importance of amino acid residues at 60 and 90 position from the N terminus of VP-TDH for. expressing biological. activity was-demonstrated.
5) By preparing chimeric toxins, importance of amino acid residues from 33 to 100 for the heat-stability of Vp-TDH was demonstrated.
6) These chimeric toxins also suggested the importance of two amino acid residues at 89 and 91 for the erythrocyte-specificity.
7) Analysis of mode of action of VP-TDH revealed that VP-TDH acts as a "preforming toxin"
8) Purification and characterization of pili produced by V. parahaemolytiucs were performed.
9) Haemagglutinins of V. parahaemolyticus was demonstrated to be a possible colonization factor of this organism,

  • Research Products

    (39 results)

All Other

All Publications (39 results)

  • [Publications] Honda,T.et al.: "Purification of a TDH-related hemolysin produced by a kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus O6:K46" FEMS Microbiol.Lett.57. 241-246 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Production of monoclonal antibodies against thermostable direct hemolyisn of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay." Med.Microbiol.Immunol.178. 245-253 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus." FEMS Microbiol.Lett.61. 95-100 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoh,M.et al.: "Homogeneity of a hemolysin(Vh-rTDH) produced by clinical and environmental isolates of Vibrio hollisae." FEMS Microbiol.Lett.61. 171-176 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishibuchi,M.et al.: "Cloning and nucleotide sequence of the gene(trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus." Infect.Immun.57. 2691-2697 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Evidence of immunologic cross-reactivity between hemolysins of Vibrio hollisae and Vibrio parahaemolyticus." J.Infect.Dis.1089-1090 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Nishibuchi,M.et al.: "Comparative analysis of the hemolysin genes of Vibrio cholerae non01,V.mimicus,V.hollisae that are similar to the tdh gene of V.parahaemolyticus." FEMS Microbiol.Lett.67. 251-256 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Production of monoclonal antibody against a hemolysin(Vp-TRH) produced by Vibrio parahaemolyticus." FEMS Microbiol.Lett.68. 167-170 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus." Can.J.Microbiol.36. 395-399 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Chracterization of a new thermostable direct hemolysin produced by a Vibrio parahaemolyticus." J.Gen.Microbiol.137. 253-259 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toda,H.et al.: "Tryptophan 65 is essential for hemolytic activity of the thermostable direct hemolysin frm Vibrio parahaemolyticus" Toxicon. 29. 834-844 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoh,M.et al.: "Characterization of thermostable direct hemolysins encoded by four representative tdh genes of Vibrio parahaemolyticus." Microb.Pathogenesis. 10. 165-172 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoshida,H.et al.: "Purification and characterization of a hemolysin of Vibrio mimicus that relates to the thermostable direct hemolysin of Vibrio parahaemolyticus." FEMS Microbiol.Lett.84. 249-254 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda,T.et al.: "Production of monoclonal antibodies against a hemagglutinin/protease of Vibrio cholerae non-01." FEMS Microbiol.Lett.78. 227-230 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arita,M.et al.: "Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus." FEMS Microbiol.Lett.79. 105-110 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Arita,M.et al.: "Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-01 serogroup hakata." Infect.Immun.59. 2186-2188 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Minami,A.et al.: "Cholera enterotoxin production in Vibrio cholerae 01 strains isolated from the environment and from humans in Japan." Appl.Environment.Microbiol.57. 2152-2157 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Yoh,M.et al.: "Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin." J.Clin.Microbiol.29. 2326-2328 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本田 武司: "腸炎ビブリオ第III集(工藤 泰雄,篠田 純男,竹田 美文,本田 武司編集)" 近代出版, 362 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本田 武司: "食中毒の正しい知識(三輪谷 俊夫監修,本田 武司,竹田 美文編集)" 菜根出版, 236 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 本田 武司,山本 耕一郎,飯田 哲也: "Medical Immunology(ビブリオ属菌の毒素,遺伝子,病原性)" 国際医書出版, 11 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Honda, T. et al.: "Purification of a TDH-related hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus O6 : K46" EFMS Microbiol. Lett.57. 241-246 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Production of monoclonal antibodies against thermostable direct hemolysin of Vibrio parahaemolyticus and application of the antibodies for enzyme-linked immunosorbent assay." Med. Microbiol. Immunol.178. 245-253 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "A mutant hemolysin with lower biological activity produced by a mutant Vibrio parahaemolyticus." FEMS Microbiol. Lett.61. 95-100 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh, M., et al.: "Homogeneity of a hemolysin (Vh-rTDH) produced by clinical and environmental isolates of Vibrio hollisae." FEMS Microbiol. Lett.61. 171-176 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishibuchi, M. et al.: "Cloning and nucleotide sequence of the gene (trh) encoding the hemolysin related to the thermostable direct hemolysin of Vibrio parahaemolyticus." Infect. Immun.57. 2691-2697 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Evidence of immunologic cross-reactivity between hemolysins of Vibrio hollisae and Vibrio parahaemolyticus." J. Infect. Dis.160. 1089-1090 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Nishibuchi, M. et al.: "Comparative analysis of the hemolysin genes of Vibrio cholerae non-O1, V. mimicus V. hollisae that are similar to the tdh gene of V. parahaemolyticus." FEMS Microbiol. Lett.67. 251-256 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Production of monoclonal antibody against a hemolysin (Vp-TRH) produced by Vibrio parahaemolyticus." FEMS Microbiol. Lett.68. 167-170 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Properties of a hemolysin related to the thermostable direct hemolysin produced by a Kanagawa phenomenon-negative clinical isolate of Vibrio parahaemolyticus." Can. J. Microbiol.36. 395-399 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Characterization of a new thermostable direct hemolysin produced by a Kanagawa-phenomenon-negative clinical isolate of Vibrio parahaemolyticus." J. Gen. Microbiol. 137. 253-259 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Toda, H. et al.: "Tryptophan 65 is essential for hemolytic activity of the thermostable directhemolysin from Vibrio parahaemolyticus." Toxicon. 29. 834-844 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh, M. et al.: "Characterization of thermostable direct hemolysins encoded by four representative tdh genes of Vibrio parahaemolyticus." Microb. Pathogenesis. 10. 165-172 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoshida, H. et al.: "Purification and characterization of a hemolysin of Vibrio mimicus that relates to the thermostable direct hemolysin of Vibrio parahaemolyticus" FEMS Microbiol. Lett.84. 249-254 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Honda, T. et al.: "Production of monoclonal antibodies against a hemagglutinin/protease of Vibrio cholerae non-OL" FEMS Microbiol. Lett.78. 227-230 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arita, M. et al.: "Purification and characterization of a heat-stable enterotoxin of Vibrio mimicus." FEMS Microbiol. Lett.79. 105-110 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Arita, M. et al.: "Purification and characterization of a new heat-stable enterotoxin produced by Vibrio cholerae non-Ol serogroup Hakata" Infect. Immun.59. 2186-2188 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Minami, A. et al.: "Cholera enterotoxin production in Vibrio cholerae Ol strains isolated from the environment and from humans in Japan." Appl. Environment. Microbiol.57. 2152-2157 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Yoh, M. et al.: "Comparison of preservation methods for enterotoxigenic Escherichia coli producing heat-labile enterotoxin" J. Clin. Microbiol.29. 2326-2328 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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