1990 Fiscal Year Final Research Report Summary
Studies on the Host Proteases which Determine Viral Spreading and Tropism.
| Project/Area Number |
01480181
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Virology
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| Research Institution | Nagoya University |
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Principal Investigator |
NAGAI Yoshiyuki Nagoya University School of Medicine, Professor, 医学部, 教授 (20022874)
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| Co-Investigator(Kenkyū-buntansha) |
HAMAGUCHI Michinari Nagoya University School of Medicine, Associate Professor, 医学部, 助教授 (90135351)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Factor X / Fusion glycoprotein / Paramyxovirus / Orthomyxovirus / Proteolytic activation / Viral tropism |
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| Research Abstract |
Host cell proteases responsible for activation of viral fusion glycoproteins are an important determinant for spread and tropism of various animal viruses. Exemplifying such proteases for the first time, we isolated an endoprotease from chick embryo that activates para- and orthomyxovirus fusion glycoproteins by cleaving their precursor proteins at a specific, single arginine-containing site. The protease is a calcium dependent serine protease consisting of two subunits, the 33 Kd catalytic chain and the 23 Kd chain possibly required for Ca^<+2> binding, and was found to be highly homologous, if not identical, to the blood clotting factor X (FX), a member of the prothrombin family. Its high efficiency and specificity 1 in cleavage reaction were attributable to the properties characteristic of FX. Its role in vivo was strongly supported by cleavage inhibition in ovo highly selective for this virus group with a specific peptide inhibitor against FX. We have further prepared specific cDNA and antibody probes for FX and are now investigating the expression of FX gene in each tissue of the whole embryo. The preliminary results obtained by these studies strongly suggested that the ectopic expression of FX is the sole determinant for the viral tropism in the embryo.
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Research Products
(14 results)
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[Publications] ASKAGUCHI,T.,TOYODA,T.,GOTOH,B.,INOCENCIO,N.M.,KUMA,K.,MIYATA,T.,and NAGAI,Y.: "Newcastle disease virus evolution.I.Mulyiple lineages defined by sequence variability of the hemagglutininーneuraminidase gene." TOYODA,T.,SAKAGUCHI,T.,Virology. 169. 260-272 (1989)
Description
「研究成果報告書概要(和文)」より
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[Publications] TOYODA,T.,SAKAGUCHI,T.,HIROTA,H.,GOTOH,B.,KUMA,K.,MIYATA,T.,and NAGAI,Y.: "Newcastle disease virus evolution.II.Lack of gene recommbination in generating virulent and avirulent strains." Virology. 169. 273-282 (1989)
Description
「研究成果報告書概要(和文)」より
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[Publications] GOTOH,B.,OGASAWARA,T.,TOYODA,T.,INOCENCIO,N.M.,HAMAGUCHI,M.,and NAGAI,Y.: "An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo." The EMBO Journal. 9. 4189-4195 (1990)
Description
「研究成果報告書概要(和文)」より
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[Publications] SAKAGUCHI, T., TOYODA, T., GOTOH, B., INOCENCIO, N. M., KUMA, K., MIYATA, T., and NAGAI, Y.: "Newcastle disease evolution. I. Multiple lineages defined by sequence variability of the hemaglutinin-neuraminidase gene." Virology. 169,. 260-272 (1989)
Description
「研究成果報告書概要(欧文)」より
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[Publications] TOYODA, T., SAKAGUCHI, T., HIROTA, H., GOTOH, B. KUMA, K., MIYATA, T., and NAGAI, Y.: "Newcastle disease virus evolution. II. Lack of gene recombination in generating virulent and avirulent strains." Virology. 169,. 273-282, (1989)
Description
「研究成果報告書概要(欧文)」より
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[Publications] INOCENCIO N. M., GOTOH, B., TOYODA, T., T., KITADA, C., and NAGAI, Y.: "Evaluaion of the antiviral effect of synthetic oligopoptides whose sequences are derived from paramyxovirus F1 N termini." Med. Microbiol. Immunol. 179,. 87-94 (1990)
Description
「研究成果報告書概要(欧文)」より
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[Publications] GOTOH, B., OGASAWARA, T., TOYODA, T., INOCENCIO, N. M., HAMAGUCHI, M. and NAGAI, Y.: "An endoprotease homologous to the blood clotting factor X as a determinant of viral tropism in chick embryo." EMBO J.9,. 4189-4195, (1990)
Description
「研究成果報告書概要(欧文)」より
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