1990 Fiscal Year Final Research Report Summary
Isolation of cDNA Clone Encodes Monoclonal Nonspecific Suppressor Factor (MNSF)
| Project/Area Number |
01480219
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
内科学一般
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| Research Institution | Shimane Medical University |
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Principal Investigator |
TSUNEMATSU Tokugoro Shimane Medical University, The Third Division of Internal Medicine, Professor, 医学部, 教授 (40026852)
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| Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Morihiko Shimane Medical University, The Third Division of Internal Medicine, Instructor, 医学部, 助手 (20155865)
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| Project Period (FY) |
1989 – 1990
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| Keywords | monoclonal / nonspecific / suppressor factor / hybridoma |
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| Research Abstract |
We obtained MNSF from supernatant of murine T cell hybridoma. MNSF was isolated with the use of anti-MNSF monoclonal antibody, following which it was further purified by reverse-phase high pressure liquid chromatography (HPLC). MNSF was analyzed by auto amino acid sequence analyzer. The N-terminal amino acid sequence data showed no homology to any known biologically active proteins including nonspecific suppressor factor.
Unfortunately, the sequence data include several ambiguous residues. Self-aggregation of MNSF also makes it difficult to isolate the cDNA. Further studies are under way to prepare MNSF gene with the use of polymelase chain reaction (PCR).
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