1990 Fiscal Year Final Research Report Summary

Isolation of cDNA Clone Encodes Monoclonal Nonspecific Suppressor Factor (MNSF)

Research Project

Project/Area Number	01480219
Research Category	Grant-in-Aid for General Scientific Research (B)
Allocation Type	Single-year Grants
Research Field	内科学一般
Research Institution	Shimane Medical University
Principal Investigator	TSUNEMATSU Tokugoro Shimane Medical University, The Third Division of Internal Medicine, Professor, 医学部, 教授 (40026852)
Co-Investigator(Kenkyū-buntansha)	NAKAMURA Morihiko Shimane Medical University, The Third Division of Internal Medicine, Instructor, 医学部, 助手 (20155865)
Project Period (FY)	1989 – 1990
Keywords	monoclonal / nonspecific / suppressor factor / hybridoma
Research Abstract	We obtained MNSF from supernatant of murine T cell hybridoma. MNSF was isolated with the use of anti-MNSF monoclonal antibody, following which it was further purified by reverse-phase high pressure liquid chromatography (HPLC). MNSF was analyzed by auto amino acid sequence analyzer. The N-terminal amino acid sequence data showed no homology to any known biologically active proteins including nonspecific suppressor factor. Unfortunately, the sequence data include several ambiguous residues. Self-aggregation of MNSF also makes it difficult to isolate the cDNA. Further studies are under way to prepare MNSF gene with the use of polymelase chain reaction (PCR).

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