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1991 Fiscal Year Final Research Report Summary

Effect of interleukin 1 and PTH related protein on hypercalcemia in adult T-cell leukemia

Research Project

Project/Area Number 01480221
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 内科学一般
Research InstitutionUniversity of Occupational & Environmental Health (U. O. E. H.)

Principal Investigator

ETO Sumiya  U. O. E. H., Medicine, Professor, 医学部, 教授 (90010347)

Co-Investigator(Kenkyū-buntansha) 白川 文彦  産業医科大学, 医学部, 講師 (10158967)
YAMASHITA Yuki  University of Hiroshima, Parasitology, Professor, 医学部, 教授 (00028680)
ODA Susumu  U. O. E. H., Medicine, Assoc. Professor, 医学部, 助教授 (80035237)
Project Period (FY) 1989 – 1991
KeywordsATL / hypercalcemia / IL1 / PTHrP / HHM / IL2 / HHM / LOH
Research Abstract

We studied about the reason why hypercalcemia is very frequently associated with adult T-cell Leukemia. The growth of adult T-cell leukemia(ATL)cells and its cell line, MT2 cells were enhanced by addition of calcium to the media in a dose dependent manner. The maximum response was induced at 4 mM calcium, which was abnormally higher than that of the normal calcium level, 2.5 mM. The production of interleukin 1(ILl), which is known to be strong bone resorption stimulating factor(BRSF), and the expression of ILl receptor was also rather enhanced in the same condition as mentioned above. These results suggest the presence of autocrine mechanism involving ILl in hypercalcemia in ATL. Namely, ILl produced by ATL cells stimulate bone resorption and induce hypercalcemia, which enhance the growth of ATL cells, ILl production and ILl receptor expression followed by higher hypercalcemia in ATL.
We also recognized that mRNA of PTH-related protein(PTHRP)which is most important BRSF in humoral hypercaleemia of malignancy(HHM)was expressed in ATL cells by northern blot hybridization assay and that C-fragment of PTHrP assayed by using RIA was increased in serum and urine of patients with ATL associated with hypercalcemia.
In conclusion, it is suggested that very high degree of complication of hypercalcemia in ATL may be induced by synergetic effect of ILl production through a kind of autocrine mechanism and PTHRP production from ATL cells.

  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Shirakawa,Fumihiko: "Calcium dependency in the growth of Adult -T cell leukemia cells in vitro." Cancer Research. 46. 658-661 (1986)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirakawa,Fumihiko: "Production of bone resorbing activity corresponding to interleukin 1α by adult T-cell leukemia cells in human." Cancer Research. 48. 4284-4287 (1988)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirakawa,Fumihiko: "Autocrine stimulation of interleukin 1α in the growth of adult human T-cell leukemia cells" Cancer Research. 49. 1143-1147 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Watanabe,Kenichi: "Interleukin-4 as a potent inhibitor of bone resorption" BBRC. 172. 1035-1041 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tanaka,Yoshiya: "Calciumdependency of the production of interleukin 1 and expression of interleukin 1 receptors of human adult T-cell leukemia cells in vitro" Cancer Research. 50. 4344-4348 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 渡辺 堅一: "RIAキットによる血清CーPTHrP測定の基礎的・臨床的検討" ホルモンと臨床. 39. 873-881 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Shirakawa, Fumihiko, et al.: "Calcium dependency in the growth of adult T-cell leukemia cells in vitro." Cancer Research. 46. 658-661 (1986)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirakawa, Fumihiko, et al.: "Production of bone resorbing activity corresponding to interleukin 1alpha by adult T-cell leukemia cells in human." Cancer Research. 48. 4284-4287 (1988)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Shirakawa, Fumihiko, et al.: "Autocrine stimulation of interleukin 1alpha in the growth of adult human T-cell leukemia cells." Cancer Research. 49. 1143-1147 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Kenichi, et al.: "Interleukin-4 as a potent inhibitor of bone resorption." B. B. R. C.172. 1035-1041 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tanaka, Yoshiya, et al.: "Calcium dependency of the production of interleukin 1 and expression of interleukin 1 receptors of human adult T-cell leukemia cells in vitro." Cancer Research. 50. 4344-4348 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Watanabe, Kenichi, et al.: "Fundamental and clinical study on assay of C-PTH related by radio-immunoassay." Clinical Endo-crinology. 39. 873-881 (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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