• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to project page

1990 Fiscal Year Final Research Report Summary

Study of Hypercholeretic and Cytoprotective Bile Acids

Research Project

Project/Area Number 01480228
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Gastroenterology
Research InstitutionNagoya City University

Principal Investigator

TAKEUCHI Toshihiko  Nagoya City University, School of Medicine Professor, 医学部, 教授 (20079990)

Co-Investigator(Kenkyū-buntansha) HAYAKAWA Tomihiro  Nagoya City University, School of Medicine Assistant, 医学部, 助手 (50172995)
KATAGIRI Kenji  Nagoya City University, School of Medicine Assistant Professor, 医学部, 講師 (50094363)
Project Period (FY) 1989 – 1990
KeywordsBile acid / Cholestasis / Cytoprotection / Primary cultured hepatocyte / Bile formation / Microtubule / βムリコ-ル酸
Research Abstract

Cytoprotective effect of ursodeoxycholic acid (UDCA) and beta-muricholic acid (beta-MCA) were examined using isolated liver perfusion and primary cultured hepatocyte of rat. In isolated liver perfusion experiments, Taurine conjugated UDCA (TUDCA) and beta-MCA (Tbeta-MCA) can support choleresis even under condition of microtubule dysfunction, while these bile acids belong to so called micelle forming bile acid. Moreover, TUDCA and Tbeta-MCA demonstrate protective effects against taurocholate induced cholestasis of colchicine-pretreated rat liver. It was additionally found that beta-MCA stimulates bicarbonate ion excretion into bile. In the experiment using primary cultured hepatocytes, Simultaneous administration of TUDCA or Tbeta-MCA with TCA decreased LDH levels in medium and reduced TCDCA content in hepatocytes. This study revealed that TUDCA and Tbeta-MCA demonstrated its cytoprotective action through the microtubule dependent choleresis and reduction of intrahepatic content of cytotoxic bile acids.

  • Research Products

    (13 results)

All Other

All Publications (13 results)

  • [Publications] 竹島 彰彦: "ビンブラスチンおよびサイトカラシンB処置ラット肝細胞におけるUrsodeoxyーcholic acid代謝について" 胆汁酸の基礎と臨床(奥山澄彦編). 23-31 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 蜂矢 仁: "Ursodeoxycholic acidによる胆汁生成の特異性についてーラット分離肝潅流法を用いての検討ー" 胆汁酸の基礎と臨床(奥山澄彦編). 32-41 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Makoto Hoshino: "Cytoskeltal Protein Changes in hepatocytes of bile ductーligated rats." Acta Histochem Cytochem. 22. 341-350 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 中井 富夫: "TC惹起胆汁うつ滞におけるタウリン抱合体UPCの防察効果ーコルヒチン処置ラットを用いてー" 肝臓. 31. 592 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 早川 富博: "胆汁脂質分泌に対するパパベリンの影響" 肝臓. 31. 1359-1360 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Tomio Nakai: "Microtabuleーindependent cholestasic and antiーcholestatic action of tauroursodeoxycholic acid." J.Lipid Res.

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Toshihiko Takeuchi edit.: "Bile Acid Metabolism in Hepatobiliary Diseases" (Kokusai Isho Shuppan Ltd.),

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Akihiko Takeshima: "Metabolism of ursodeoxycholic acid in vinblatine or cytochalacin B -treasted rat hepatocyte (Japanese)" Clinical research of bile acid. 23-31 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Hitishi Hachiya: "Characteristic bile formation by urso-deoxycholic acid-using isolated rat liver perfusion experiment-(Japanese)" Clinical research of bile acid. 32-41 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomio Nakai: "Protective effect of taurine conjugated UDCA against TC-induced cholestasis-" Acta Hepatologica Jpn. 31. 592 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kenji Katagiri: "The preventive effects of beta-muri-cholic acid and tauro-beta-muricholic-acid against taurocholate-induced cholestasis in colchicine-pretreated rat liver" Gastroenterology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Takayuki Ohoiwa: "Reduction of intrahepatocellular levels of taurodeoxycholic acid band hepatotoxity by tauro-urso deoxycholate and tauro-betamuricholate" Gastroenterology.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tomio Nakai: "Microtuble-independent choleretic and anti-cholestatic action of tauro-ursodeoxycholic acid" J Lipid Res.

    • Description
      「研究成果報告書概要(欧文)」より

URL: 

Published: 1993-08-12  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi