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1991 Fiscal Year Final Research Report Summary

Basic and Clinical Studies on Humoral Control of Sleep

Research Project

Project/Area Number 01480279
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Psychiatric science
Research InstitutionShiga University of Medical Science

Principal Investigator

KATO Nobumasa  Shiga Univ Med Sci, Dept of Psychiatry, Associate Professor, 医学部, 助教授 (10106213)

Co-Investigator(Kenkyū-buntansha) TSUNASHIMA Koichi  Natl Centre Neurol Psychiat, Div Men Tal Dis Res, Res Fellow, 神経センター・神経研究所, 研究員 (30197743)
IIDA Hideharu  Shiga Univ Med Sci, Dept of Psychiarty, Assistant, 医学部, 助手 (50184354)
Project Period (FY) 1989 – 1991
KeywordsDSIP / Dopamine / Serotonin / Growth hormone / Thyroid-stimulating hormone / Prolactin / Circadian rhythm / Thermoregulation
Research Abstract

The humoral control of sleep mechanisms was investigated in terms of 1) possible roles of delta sleep-inducing peptide (DSIP) as one of slow-wave promoting substances, and 2) secretary pattern of growth hormone (GH) during slow wave sleep (SWS).
A sensitive enzyme immunoassay (EIA) method for measuring DSIP in body fluids was developed in the laboratory. This method was unable to detect DSIP-like material in human and rat tissues. Plasma DSIP levels were measured throughout 24 h in healthy male subjects and diurnal variations of circulatory DSIP concentrations were found. Besides its known sleep-promoting effect, we found the thermoregulatory action of DSIP in rats. DSIP as well as its phosphorylated form (P-DSIP) was found to potentiate dopamine- or serotonin-induced hypothermia in rats at the ambient temperature. Pharmacological study showed this thermoregulatory action of DSIP was exerted via 5-HT 1A receptors. Zopiclone, one of non-benzodiazepine hypnotics was demonstrated to increa … More se DSIP contents in the rat brain dose-dependently. These findings may further present significant roles of DSIP in modulating sleep and sleep-related phenomena.
An ultra-sensitive EIA method of human GH was introduced to evaluate 24 h secretary pattern in normal condition and in sleep-deprived condition. The lowest level of basal plasma GH was as low as 10 pg/ml, which could be detected for the first time by this method. Twenty-four hour secretory rhythm of GH in normal and delayed sleep phase suggested the presence of ultradian rhythm in GH secretion. Prolactin and thyroid-stimulating hormone (TSH) were also measured in the same samples. Prolactin was found to be elevated during sleep and prolactin secretion appeared to be promoted after REM sleep. Furthermore, the relationship between hormone secretion and initiation of the first SWS was studied in more detail by frequent determinations of plasma GH and prolactin in 5-min intervals. GH secretion increased 10 min after the initiation of SWS, and the pattern of prolactin secretion was found to be almost identical with GH secretion. Namely, GH secretion, prolactin secretion and the first SWS processes were somehow synchronized one another with a short delay. TSH secretion had its own secretory pattern with higher values during night, which normally was inhibited by sleep. Less

  • Research Products

    (22 results)

All Other

All Publications (22 results)

  • [Publications] 定松 美幸,加藤 進昌: "睡眠をひきおこす「睡眠物質」とは" こころの臨床ア・ラ・カルト. 8(4). 13-17 (1989)

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      「研究成果報告書概要(和文)」より
  • [Publications] 定松 美幸,飯田 英晴,加藤 進昌,橋田 誠一,石川 栄治: "高感度酵素免疫測定法によるヒト成長ホルモン分泌リズムの睡眠依存性の再検討" 脳と精神の医学. 1. 21-27 (1990)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tsunashima K,Masui A,and Rato N.: "The effect of delta sleep-inducing pepride(DSIP)and phosphorylated DSIP(P-DSIP)on the apomorphine-induced hypothermia in rats." Brain Research. 510. 171-174 (1990)

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      「研究成果報告書概要(和文)」より
  • [Publications] 飯田 英晴,定松 美幸,加藤 進昌: "ヒト・プロラクチン分泌リズムの睡眠依存性ーレム・ソンレム周期との相関についてー" 脳と精神の医学. 2. 265-271 (1991)

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  • [Publications] 加藤 進昌,定松 美幸,飯田 英晴,増井 晃,高橋 三郎: "睡眠とGrowth hormone(GH)ーGH分泌の睡眠依存性とデルタ睡眠誘発ペプチド(DSIP)ー" ホルモンと臨床. 39. 585-590 (1991)

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  • [Publications] 加藤 進昌,定松 美幸: "睡眠異常の内分泌学的アプロ-チ" クリニカル、ニュ-ロサイエンス. 9. 1062-1065 (1991)

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      「研究成果報告書概要(和文)」より
  • [Publications] 綱島 浩一,山寺 博史,加藤 進昌,ミツシタ 洋,高橋 清久: "ゾピクロン投与によるラット脳内および血中デルタ睡眠誘発ペプチド様免疫活性(DSIPーLI)の変化" 脳と精神の医学. 3. (1992)

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      「研究成果報告書概要(和文)」より
  • [Publications] Tsunashima K,Kato N,Masui A,& Takahashi K: "The effect of delta stecp-inducing peptide(DSIP)on the changes of body(core)temperature induced by serotonergic agonists in the rats." Peptides.

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      「研究成果報告書概要(和文)」より
  • [Publications] 定松 美幸,飯田 英晴,加藤 進昌,高橋 三郎,橋田 誠一,石川 栄治: "ヒト成長ホルモンとプロラクモンの入眠時徐波睡眠依存性の再検討" 日本内分泌学会雑誌.

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      「研究成果報告書概要(和文)」より
  • [Publications] Kato N,Nagaki S,Someya T,Tsunashima K,Kawata E,Masui A,Sadamatsu M and Iida H.: "DSIP and the ciacadian sleep-wake rhythm In "Endogenous Sleep Factors"(eds.by Inone S.and Krueger JM)" Bouma Text Wasseuaar Amsterdam, 175-184(321) (1990)

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  • [Publications] 綱島 浩一,加藤 進昌: "ストレスと神経内分泌 In:生物学的精神医学シリ-ズ第3巻「躁うつ病と神経内分泌」(野村・前田編)P'F収" 学会出版センタ-,東京, (1992)

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  • [Publications] Sadamatsu M. et al.: "Sleep-promoting substance." Kokoro-no-Rinshou. 8(4). 13-17 (1989)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Sadamatsu M. et al.: "Re-evaluation of sleep dependency of secretory rhythm of human growth hormone using highly sensitive enzyme immunoassay." Brain Sci Ment Dis. 1. 21-27 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunashima K. et al.: "The effect of delta sleep-inducing peptide (DSIP) and phosphorylated DSIP (P-DSIP) on the apomorphine-induced hypothermia in rats." Brain Research. 510. 171-174 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Iida H. et al: "Sleep dependency of the secretory rhythm of human prolactin on the correlation with REM-NREM cycles." Brain Sci Ment Dis. 2. 265-271 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Kato N. et al.: "Sleep and growth hormone (GH) : sleep dependency of GH secretion and 24-h secretory rythm of delta sleep-inducing peptide (DSIP)." Horumon-to-Rinshou. 39. 585-590 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kato N. et al.: "Endocrinological approach to sleep disorders." Clinical Neuroscience. 9. 1062-1065 (1991)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunashima K. et al.: "Changes of delta sleep-inducing peptide-like immunoreactivity (DSIP-LI) in rat brain and plasma following treatment with zopiclone." Brain Sci Ment Dis. 3. (1992)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunashima K. et al.: "The effect of delta sleep-inducing peptide (DSIP) on the changes of body (core) temperature induced by serotonergic agonists in the rats." Peptides.

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Sadamatsu M. et al.: "Correlation of secretory patterns of human growth hormone and prolactin with the first slow wave sleep." Folia Endocrinol Jap.

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      「研究成果報告書概要(欧文)」より
  • [Publications] Kato N. et al.: "DSIP and the circadian sleep-wake rhythm." Endogenous Sleep Factors. Bouma Text Wassenaar. 175-184 (1990)

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      「研究成果報告書概要(欧文)」より
  • [Publications] Tsunashima K. et al.: "Stress and neuroendocrinology." Manic-depressive Illness and Neuroendocrinology. Japan Sci Soc Press.

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Published: 1993-03-16  

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