1990 Fiscal Year Final Research Report Summary
Analysis and suppression of Graft-versus-Host (GVH) reaction after bone marrow transplantation
| Project/Area Number |
01480296
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Hematology
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| Research Institution | University of Tokyo |
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Principal Investigator |
MINAMI Matsuhiko University of Tokyo, Dept Transfusionn Medicine, Associate Professor, 医学部(病), 助教授 (60092342)
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| Co-Investigator(Kenkyū-buntansha) |
TAKIGUCHI Masafumi University of Tokyo, Dept Immunology, Assistant Professor, 医科学研究所, 助手 (00183450)
JUJI Takeo University of Tokyo, Dept Transfusionn Medicine, Professor, 医学部(病), 教授 (20009997)
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| Project Period (FY) |
1989 – 1990
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| Keywords | GVH / MHC / minor histocompatibility / CTL / HLA / T cell receptor / alloreactive T cell |
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| Research Abstract |
The project suppored by this grant intended to clarify mechanisms of induction of graft-versus-host (GVH) diseases. The clarification is required for establishment of methods for prevention or therapy of GVH diseases. We did the following subjects ; 1) analysis of major histocompatibility complex (MHC) molecules, especially HLA class I molecules, 2) analysis of minor histocompatibility (mH) antigenic system in human, and 3) finally suppression of alloreactive T cells inducing GVH diseases. First, we analyzed molecular structure and molecular evolution on HLA-B51 and B52, HLA-B35 and HLA-C molecules. Further, from the peripheral blood lymphocytes of a patient with kidney transplantation, we established cytotoxic T cell clones responding to a type of mH antigen on the cells of the patient. The CTL clones could kill HLA-B35-positive cells without expression of the type of mH antigen in the presence of the peptide (s) eluted from the cells of the patient. Also, from the transfused patient we established T cell clone reacting to mH antigen in a HLA-DR9-restricted manner. Finally, in the serum of a multitransfused patient we detected and characterized antibodies reacting to T cell receptors expressed on the T cells reacting to allo-HLA expressed on the patient.
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Research Products
(14 results)
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[Publications] Yamamoto,J.,Hiraiwa,M.,Hayashi,H.,Tanabe,M.,Kano,K.,and Takiguchi,M.: "Two amino acid substitutions at residues 63 and 67 between HLA-B51 and HLA-Bw52 from multiple epitopes recognized by allogeneic T cells." Immunogenetics. 33. 286-289 (1991)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nishimura,M.,Sakai,K.,Akaza,T.,Mitomi,Y.,Nieda,M.,Minami,M.,and Juji,T.: "Anti-idiotypic antibody to T cell receptor in multiply transfused patients may play a role in resistance to graft-versus-host disease." Transfusion. 32. 719-728 (1992)
Description
「研究成果報告書概要(和文)」より
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[Publications] Nishimura,M.,Sakai,K.,Akaza,T.,Mitomi,Y.,Nieda,M.,Minami,M.,and Juji,T.: "Establishiment of human minor histocompatibility antigen-specific cytotoxid T cell clones restricted by HLA-DR9." Transplantation. 55. 1181-1186 (1993)
Description
「研究成果報告書概要(和文)」より
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[Publications] Yamamoto, J., Hiraiwa, M., Hayashi, H., Tanabe, M., Kano, K., and Takiguchi, M.: "Two amino acid substitutions at residues 63 and 67 between HLA-B51 and HLA-Bw52 from multiple epitopes recognized by allogeneic T cells." Immunogenetics. 1991.33. 286-289 (1991)
Description
「研究成果報告書概要(欧文)」より
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[Publications] Nishimura, M., Sakai, K., Akaza, T., Mitomi, Y., Nieda, M., Minami, M., and Juji, T.: "Anti-idiotypic antibody to T cell receptor in multiply transfused patients may play a role in resistance to graft-versus-host disease." Transfusion. 32. 719-728 (1992)
Description
「研究成果報告書概要(欧文)」より
-
[Publications] Nishimura, M., Sakai, K., Akaza, T., Mitomi, Y., Nieda, M., Minami, M., and Juji, T.: "Establishment of human minor histocompatibility antigen-specific cytotoxic T cell clones restricted by HLA-DR9." Transplantaion. 55. 1181-1186 (1993)
Description
「研究成果報告書概要(欧文)」より
