1990 Fiscal Year Final Research Report Summary
Preclinical Study of Radioimmunoguided Surgery Using Metastases Model of Human Colon Cancer in Congenitally Athymic mice.
| Project/Area Number |
01480334
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| Research Category |
Grant-in-Aid for General Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Research Field |
Digestive surgery
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| Research Institution | The Jikei University School of Medicine |
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Principal Investigator |
SAKURAI Kenji The Jikei Univ. School of Med. 1st Dept. of Surgery, Professor, 医学部・第一外科, 教授 (00147315)
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| Co-Investigator(Kenkyū-buntansha) |
UNEMURA Yasuki The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (00193920)
YOSHIDA Kazuhiko The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (90191577)
ISHIDA Shusei The Jikei Univ. School of Med. 1st Dept. of Surgery, Assistant, 第一外科, 助手 (60168226)
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| Project Period (FY) |
1989 – 1990
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| Keywords | Monoclonal antibody / Radioimmunoguided surgery / Colon cancer / Hepatic metastases / Tumor specific antigen |
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| Research Abstract |
Reproducible murine metastases models in the liver, lung, and peritoneum from human colon cancer (HCC) were developed in congenitally athymic mice, and the utility of a gamma detecting probe (GDP) in the detection of hepatic metastases using radiolabeled monoclonal antibody (mAb) was assessed.
One hundred percent of mice developed hepatic metastases after intrasplenic injection of HT29LMM HCC cells, and 100% also had peritoneal metastases after intraperitoneal injection. Additionally, 60% of mice developed lung metastases after tail vein injection.
Mice with hepatic metastases were injected intravenously with 40 mu Ci/4mugof I-125 labeled mAb HT 29-15 and an isotype-matched control mAb (BL-3). And non-tumor-bearing control mice were injected with the same dose of I-125 labeled mAb HT29-15. Using GDP, measurements were obtained daily over the region of the heart, the region of the liver (the right flank). External right flank/heart ratios were significantly higher in the tumor-bearing group with mAb HT-29-15 than in controls. External measurements allowed detection of small tumors weighing only 161mg. Intraoperative measurements of small isolated metastasis were obtained at laparotomy on days 5 and 7. The mean metastasis/heart GDP ratio count was 1.7, and tumors as small as 51mg in weight could be identified.
These experimental results confirm the usefulness of the GDP for the detection of small hepatic metastases from colon cancer, and illustrate important features of probe measurement of radiolabeled mAb uptake.
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