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1990 Fiscal Year Final Research Report Summary

Study on Molecular Biological Control of Human Testicular Function

Research Project

Project/Area Number 01480382
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field Urology
Research InstitutionOsaka University

Principal Investigator

NAMIKI Mikio  Assistant Professor, Faculty of Medicine Osaka University, 医学部, 助手 (70155985)

Co-Investigator(Kenkyū-buntansha) NAKAMURA Masahiro  Assistant Professor, Osaka University, 医学部, 助手 (60135689)
OKUYAMA Akihiko  Associate Professor, Osaka University, 医学部, 助教授 (20093388)
Project Period (FY) 1989 – 1990
KeywordsAndrogen / Receptor / Infertility / Hypogonadism / Leydig cell / DNA
Research Abstract

1. To evaluate the effect of hMG-hCG treatment on spermatogenetic impairment, DNA/RNA synthesis were measured in 25 patients with idiopathic male infertility by estimating the values of ^3H-thymidine and ^<14>C-uridine incorporation into the testicular tissue cultured with or without the hormonal treatment. It would appear that the DNA synthesis in testicular tissue cultured with or without hMG-hCG treatment might predict the responsiveness to hormonal treatment for patients with idiopathic male infertility.
2. To study the effectiveness of hCG and hMG administration on spermatogenesis of patients with hypogonadotropic hypogonadism (HH), the DNA synthesis of spermatogonia was investigated by the method of ^3H-thymidine uptake by cultured tissue fragments of human testis. Combined hCG and hMG therapy was effective for induction of spermatogenesis in patients with HH.
3. Localization of androgen receptors (ARs) in the human testis Leydig cells was examined with an AR assay and Northern blot analysis. ARs in Leydig cells showed a high affinity for ( ^3H)methyltrienol and the Kd and Bmax of the receptors were 1.24 nM and 11.7 fmol/mg protein, respectively. Northern blot analysis, using a ^<32>p-labeled full-length human AR complementary DNA (cDNA) detected a 9.5-kb hybridizing band in the total RNA extracted from Leydig cells. These data can be interpreted as evidence of the existence of ARs in human Leydig cells.

  • Research Products

    (8 results)

All Other

All Publications (8 results)

  • [Publications] A.Okuyama,N.Nonomura,M.Nakamura,M.Namiki,E.Koh et al.: "Effectiveness of hMGーhCG treatment for DNA/RNA synthesis in subfertile human testis in vitro" Archeves of Andrology. 22. 167-172 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A.Okuyama,N.Nonomura,E.Koh,N.kondoh,M.Nakamura,M.Namiki et al.: "Induction of testicular DNA synthesis in vitro by hCG and hMG treatments in men with hypogonadotropic hypogonadism" Archives of Andrology. 23. 39-43 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Namiki,K.Yokokawa,A.Okuyama,E.Koh,H.Kiyihara et al.: "Evidence for the presence of androgen receptors in human Leydig cells" Journal of Steroid Biochemistry and Molecular Biology. 38. 79-82 (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] M.Namiki,E.Koh,K.Yokokawa,A.Okuyama,N.Kondoh et al.: "Androgen receptors in human Leydig cells" Molecular Andrology. 3. (1991)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] A. Okuyama, N. Nonomura, M. Nakamura, M. Namiki, E. Koh, N. Kondoh, H. Kiyohara, Y. Nishimune, K. Matsumoto, T. Sonoda.: "Effectiveness of hMG-hCG treatment for DNA/RNA synthesis in subfertile human testis in vitro." Archives of Andrology. Vol 22. 167-172 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] A. Okuyama, N. Nonomura, E. Koh, N. Kondoh, M. Nakamura, M. Namiki, H. Kiyohara, Y. Nishimune, K. Matsumoto, M. Matsuda, T. Sonoda.: "Induction of testicular DNA synthesis in vitro by hCG and hMG treatments in men with hypogonadotropic hypogonadism." Archives of Androgyny. Vol 23. 39-43 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Namiki, K. Yokokawa, A. Okuyama, E. Koh, M, Nakao, S. Sakoda, K. Matsumoto, T. Sonoda.: "Evidence for the presence of androgen receptors in human Leydig cells." Journal of Steroid Biochemistry and Molecular Biology. Vol 38. 79-82 (1991)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] M. Namiki, E. Koh, K. Yokokawa, A. Okuyama, N. Kondoh, H. Kiyohara, S. Sakoda, K. Matsumoto, T. Sonoda.: "Androgen receptors in human Leydig cells." Molecular Androgyny. Vol 3. (1991)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-08-12  

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