Serological and Histochemical Study of Tumor Markers on DMBA Induced Lingual Carcinoma

1990 Fiscal Year Final Research Report Summary

• Project/Area Number: 01480470
• Research Category: Grant-in-Aid for General Scientific Research (B)
• Allocation Type: Single-year Grants
• Research Field: 外科・放射線系歯学
• Research Institution: Okayama University
• Principal Investigator: KISHI Kanji Okayama University Dental School, Professor, 歯学部, 教授 (30033202)
• Co-Investigator(Kenkyū-buntansha): FURUTA Hiroaki Okayama University Dental School, Professor, 歯学部, 教授 (30018428) ; HIRAKAWA Kouichi Okayama University Dental School, Research Assistant, 歯学部, 助手 (70189827) ; AKITA Kazutoshi Okayama University Dental School, Research Assistant, 歯学部, 助手 (10167835)
• Project Period (FY): 1989 – 1990
• Keywords: Tongue cancer / Tumor maker / Radiation / Histochemistry / Medication / Serology

Research Abstract

There was a significant increase in the concentrations of putrescine, cadaverine and spermidine in the cancer tissue. The concehtrations of polyamines were decreased by X-radiation. There were no difference in the concentrations of polyamines in the cancer tissues after the administration of Cephalanthin as compared with that of the control. Therefore, the antitumor activity of Cephalanthin was not recognized. The decrease of the concentrations of polyamines in the tumor tissue by radiation effect was enhanced when combined with the administration of Cepharanthin. Induction HDC and ODC activity (Synthesizing enzymes of histamine and putrescine) was slight by the single administration of actinomycine D and by the painting with DMBA. But the induction of HDC activity was markedly increased with the combined application of DMBA and actinomycine D.
TPA・BI antibody, was applied to immunohistochemistry to establish the method to detect the irradiation effect on DMBA-induced tongue squamous ce ll carcinomas in hamsters. Besides, the correlation between TPA・BI antibody and PKK-1, which were considered to be related to the carcinogenesis and the degree of the malignant progress was examined.
Serum TPA values of the hamsters tended to increase in the group with carcinoma though the serum TPS values had no significant differences between the pre- and post- irradiation time.
In the correlation between TPA・B1 antibody and PKK-1, biochemical analysis revealed that both TPA・B1 antibodies and PKK-1 reacted only with cytokeratin subunit No.18 (45kd) in the normal epitheliums and the squamous cell carcinomas of hamster tongues. Immunohistochemically, TPA・B1 antibodies were poitive at proliferating area of normal epitheliums and squamous cell carcinomas of hamster tongues, which was corresponded with the staining of PKK-1.
In the cases of irradiated carcinomas, the cells with positive for TPA・B1 antibody were also positive of the anti-BrdU antibody, and the necrotic tissues were negative for both antibodies. Therefore, the area positive for TPA・B1 antibody was considered to be correlated with the proliferation of the tumor cells.
As a result, TPA・B1 antibody, which has been used for the serological marker, was also considered to be useful to detect the histopathological changes of the tumor tissues caused by irradiation with single application of TPA・B1 antibody or combined with anti-BrdU antibody.