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1991 Fiscal Year Final Research Report Summary

Fundamental studies toward molecular biology of highly radiation-sensitive cells in intestinal epithelium

Research Project

Project/Area Number 01480532
Research Category

Grant-in-Aid for General Scientific Research (B)

Allocation TypeSingle-year Grants
Research Field 放射線5生物学
Research InstitutionUniversity of Tokyo

Principal Investigator

IJIRI Kenichi  Tokyo Univ., Radioisotope Center, Associate Professor, アイソトープ総合センター, 助教授 (40111447)

Co-Investigator(Kenkyū-buntansha) NOGAWA Norio  Tokyo Univ., Radioisotope Center, Technical staff, アイソトープ総合センター, 教務職員 (60208311)
TAO Kazuyuki  Tokyo Univ., Radioisotope Center, Assistant, アイソトープ総合センター, 助手 (00211996)
Project Period (FY) 1989 – 1991
KeywordsStem cells / Intestine / Crypt / Cell death / Apoptosis / Radiation / Radiosensitivity / Hypersensitive cells
Research Abstract

A technique for separating mouse small intestinal epithelial cells in vitro was established. The technique employed the mechanical and chemical separation methods together, and the validity of this technique was checked using the small intestine of the mice injected with I-125 deoxyuridine. Then, repeated injections of tritiated (H-3) thymidine were carried out from 18 hours till the 5th day after 10 Gy, and. at the points from half a month to 4 months later mice were sacrificed. Thirty minutes before the sacrifice, C-14 thymidine was injected into mice, their small intestines were excised, and cells were separated using the separation technique mentioned above. The radioactivity of H-3 and C-14 of each separated fractions were measured by the liquid scintillation counter. The fraction of the peak H-3 count and that of C-14 count slightly differed, showing that the (long-lived) stem cell population and rapidly-cycling cell population are the two different populations in the intestinal epithelium. Cell death (apoptosis) induced by a small dose of gamma rays were also examined in the intestinal crypts, of which stem cells were labelled as above and left for 0.5 to 6 months. The autoradiographs of the crypts cells were also made. The results clearly revealed that the stem cells and the target cells for apoptosis (hypersensitive cells) are different cell populations.

  • Research Products

    (11 results)

All Other

All Publications (11 results)

  • [Publications] Ijiri,K.: "Cell death (apoptosis)in mouse intestine after continuous irradiation with γ rays and with β rays from tritiated water" Radiation Research. 118. 180-191 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ijiri,K.: "Apoptosis (cell death) induced in mouse bowel by 1,2-dimethy1-hydrazine,methylazoxymethanol acetate,and γ-rays" Cancer Research. 49. 6342-6346 (1989)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ijiri,K.: "The circadian rhythm for the number and sensitivity of radiation-induced apoptosis in the crypts of mouse small intestine" International Journal of Radiation Biology. 58. 165-175 (1990)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ijiri,K.: "Circadian rhythms in the sensitivity to radiation and different drugs in mouse intestinal epithelium" Proceedings of 3rd International Symposium on Space Medicine. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] 井尻 憲一: "細胞社会における生と死" 東京大学公開講座「生と死」. 54. (1992)

    • Description
      「研究成果報告書概要(和文)」より
  • [Publications] Ijiri, K.: "Cell death (apoptosis) in mouse intestine after continuous irradiatiion with gamma rays and with beta rays from tritiated water." Radiation Research. 1.118. 180-191 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ijiri, K.: "Apoptosis (cell death) induced in mouse bowel by 1, 2-dimethylhydrazine, methylazoxymethanol acetate, and gamma -rays." Cancer Research. 49. 6342-6346 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ijiri, K.: "Cell death induced in mouse intestine by tritiated water, tritiated thymidine and gamma rays." Proceedings of the 3rd Japan-US Workshop on Tritium Radiobiology and Health Physics. IPPJ-REV-3. 217-222 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Morikawa, N., Ijiri, K., Shiroya, T., Okada, S., Nakamura, N., Ohno, M. and Nogawa, N.: "Safe-handling of tritiated water - hood, monitoring and inventory -." Proceedings of the 3rd Japan-US Workshop on Tritium Radiobiology and Health Physics. IPPJ-REV-3. 129-136 (1989)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ijiri, K. and Potten, C. S.: "The circadian rhythm for the number and sensitivity of radiation-induced apoptosis in the small intestinal crypts of mouse." Int. J. Radiat. Biol.58. 165-175 (1990)

    • Description
      「研究成果報告書概要(欧文)」より
  • [Publications] Ijiri, K.: "Circadian rhyrhms in the sensitivity to radiation and different drugs in mouse intestinal epithelium." Proceedings of ISSM'92, Nagoya. (1992)

    • Description
      「研究成果報告書概要(欧文)」より

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Published: 1993-03-16  

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